| Summary: | Erythromycin is a secondary metabolites, which is producted by
Saccharopolyspora erythraea fermentation. Erythromycin included in macrolide
group that has activities as bactericidal and bacteriostatic against Gram positives
bacteries.
The purpose of this research is to gather the best composition of media
by adding some materials on standard media of erythromycin fermentation so that
can increase the production of erythromycin. This research can be done by doing
some addition of materials such as cooking oil�s waste which is being variated on
concentration start from 1 until 5% in standard media that had been added by
molasses 1% and tofu�s waste 1%. There are some steps in this research, such as
regeneration, inoculation and fermentation, and also sample testing. The
fermentation can be done within 7 days on 28�C, 180 rpm. Sampling performed
during 7 days, start from the first day until the seventh day. Sample was
sentrifugated to separate between biomass and supernatan. Sample testing that
were performed was measurement of the growth profile, measurement of
inhibitory diameter and also measurement of glucose from the sample.
The biomass was used for measuring the growth profile of Sac. erythraea
BM1/A13 wiht PMV method (Packed Mycelia Volume). With this method, can
be known that the growth from sample faster than the control and the number of
cells much more than control. Supernatan was used for measuring the activity by
inhibitory diameter of erythromycin. B1 sample can producted erythromycin as
much as 255,3% which is much more than control, so that the addition of
materials on the media can increase the erythromycin production. Moreover, there
was no remained glucose in the end of fermentation. It means all the glucose was
used by Sac. erythraea for growing and also for producing the erythromycin.
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