VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV
Coffe powder baverages in sachet contained caffeine, aspartame, and potassium acesulfame were widely spread and consumed by public. but there were no analytical methods to measure caffeine, aspartame and potassium acesulfame in those product using HPLC equiped by UV detector. Aims of this study was...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1797034859021991936 |
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| author | , Mohamad Bagir , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_facet | , Mohamad Bagir , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_sort | , Mohamad Bagir |
| collection | UGM |
| description | Coffe powder baverages in sachet contained caffeine, aspartame, and
potassium acesulfame were widely spread and consumed by public. but there were
no analytical methods to measure caffeine, aspartame and potassium acesulfame
in those product using HPLC equiped by UV detector. Aims of this study was to
obtain high performance liquid chromatography method that met the validation
parameters included selectivity, linearity, accuracy, precision, limit of detection
and limit of quantitation, and robustness to determine concentration of those
analytes in coffe powder baverages samples.
The HPLC system consisted of Waters Atlantis dC18 (lenght 150 mm,
internal diameter 3,6 mm, particle size 5 μm) as a stationary phase, the mobile
phase is a mixtures of 25 mM phosphat buffer pH 3,5 with 20% of etanol, with a
flow rate of 1,0 mL/menit, detected by UV detector, operating time of 10 minutes
and 30oC as operating temperature.
The method validation showed a good selectivity. Linearity range were
8 - 28 mg/L for potassium acesulfame and aspartame, while 50 - 200 mg/L mg/L
for caffeine, with correlation coefficient (r) > 0.995 for all analytes. Recoveries of
the analytes from spiked energy drink samples were between 90 - 107% for
potassium acesulfame and aspartame and 95 - 105% for caffein. Nevertheles,
robustness showed this method had not a good robust. |
| first_indexed | 2024-03-13T23:18:34Z |
| format | Thesis |
| id | oai:generic.eprints.org:127838 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:18:34Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1278382016-03-04T08:02:17Z https://repository.ugm.ac.id/127838/ VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV , Mohamad Bagir , Prof. Dr. Sudibyo Martono, M.S., Apt. ETD Coffe powder baverages in sachet contained caffeine, aspartame, and potassium acesulfame were widely spread and consumed by public. but there were no analytical methods to measure caffeine, aspartame and potassium acesulfame in those product using HPLC equiped by UV detector. Aims of this study was to obtain high performance liquid chromatography method that met the validation parameters included selectivity, linearity, accuracy, precision, limit of detection and limit of quantitation, and robustness to determine concentration of those analytes in coffe powder baverages samples. The HPLC system consisted of Waters Atlantis dC18 (lenght 150 mm, internal diameter 3,6 mm, particle size 5 μm) as a stationary phase, the mobile phase is a mixtures of 25 mM phosphat buffer pH 3,5 with 20% of etanol, with a flow rate of 1,0 mL/menit, detected by UV detector, operating time of 10 minutes and 30oC as operating temperature. The method validation showed a good selectivity. Linearity range were 8 - 28 mg/L for potassium acesulfame and aspartame, while 50 - 200 mg/L mg/L for caffeine, with correlation coefficient (r) > 0.995 for all analytes. Recoveries of the analytes from spiked energy drink samples were between 90 - 107% for potassium acesulfame and aspartame and 95 - 105% for caffein. Nevertheles, robustness showed this method had not a good robust. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Mohamad Bagir and , Prof. Dr. Sudibyo Martono, M.S., Apt. (2014) VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68143 |
| spellingShingle | ETD , Mohamad Bagir , Prof. Dr. Sudibyo Martono, M.S., Apt. VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title | VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title_full | VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title_fullStr | VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title_full_unstemmed | VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title_short | VALIDASI METODE PENETAPAN KADAR KALIUM ASESULFAM, KAFEIN DAN ASPARTAM DALAM SEDIAAN MINUMAN SERBUK KOPI SACHET SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN DETEKTOR UV |
| title_sort | validasi metode penetapan kadar kalium asesulfam kafein dan aspartam dalam sediaan minuman serbuk kopi sachet secara kromatografi cair kinerja tinggi dengan detektor uv |
| topic | ETD |
| work_keys_str_mv | AT mohamadbagir validasimetodepenetapankadarkaliumasesulfamkafeindanaspartamdalamsediaanminumanserbukkopisachetsecarakromatograficairkinerjatinggidengandetektoruv AT profdrsudibyomartonomsapt validasimetodepenetapankadarkaliumasesulfamkafeindanaspartamdalamsediaanminumanserbukkopisachetsecarakromatograficairkinerjatinggidengandetektoruv |