Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli
Probes for detection of betaine genes have been created by PCR approach using genomic DNA of Escherichia coli as template. Primers were designed to amplify both betA and betB-encoding DNA sequences. The PCR fragments were then cloned into pUC19 and subsequently labeled with digoxigenin. The probes h...
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[Yogyakarta] : Universitas Gadjah Mada
1996
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