Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli
Probes for detection of betaine genes have been created by PCR approach using genomic DNA of Escherichia coli as template. Primers were designed to amplify both betA and betB-encoding DNA sequences. The PCR fragments were then cloned into pUC19 and subsequently labeled with digoxigenin. The probes h...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
1996
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| _version_ | 1797017617867735040 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Probes for detection of betaine genes have been created by PCR approach using genomic DNA of Escherichia coli as template. Primers were designed to amplify both betA and betB-encoding DNA sequences. The PCR fragments were then cloned into pUC19 and subsequently labeled with digoxigenin. The probes have been succesfrdly used for hybridisation with genomic DNA of E. coli. Following hybridisation with genomic DNA of rhizobacteria, however, no significant signal has been detected. The possible factors contributing to this failure of detection is discussed.
Key words: Betaine - rhizobacteria - probe |
| first_indexed | 2024-03-13T18:33:11Z |
| format | Article |
| id | oai:generic.eprints.org:17538 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T18:33:11Z |
| publishDate | 1996 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:175382014-06-18T00:38:28Z https://repository.ugm.ac.id/17538/ Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli Perpustakaan UGM, i-lib Jurnal i-lib UGM Probes for detection of betaine genes have been created by PCR approach using genomic DNA of Escherichia coli as template. Primers were designed to amplify both betA and betB-encoding DNA sequences. The PCR fragments were then cloned into pUC19 and subsequently labeled with digoxigenin. The probes have been succesfrdly used for hybridisation with genomic DNA of E. coli. Following hybridisation with genomic DNA of rhizobacteria, however, no significant signal has been detected. The possible factors contributing to this failure of detection is discussed. Key words: Betaine - rhizobacteria - probe [Yogyakarta] : Universitas Gadjah Mada 1996 Article NonPeerReviewed Perpustakaan UGM, i-lib (1996) Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=297 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title | Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title_full | Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title_fullStr | Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title_full_unstemmed | Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title_short | Development of probes for detection of betaine genes in rhizobacteria using PCR-amplified betaine-encoding DNA sequences of Escherichia coli |
| title_sort | development of probes for detection of betaine genes in rhizobacteria using pcr amplified betaine encoding dna sequences of escherichia coli |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib developmentofprobesfordetectionofbetainegenesinrhizobacteriausingpcramplifiedbetaineencodingdnasequencesofescherichiacoli |