Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.

HBV replicates within the nucleus of hepatocyte using cellular transport machinery for the import of their genomes into the nucleus. Genome of HBV has to transported through the cytoplasm towards the nuclear pore complex (NPC) followed by subsequent passage through the pore. HBV capsid is involved i...

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Main Author:	Perpustakaan UGM, i-lib
Format:	Article
Published:	[Yogyakarta] : Universitas Gadjah Mada 2006
Subjects:	Jurnal i-lib UGM

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author	Perpustakaan UGM, i-lib
author_facet	Perpustakaan UGM, i-lib
author_sort	Perpustakaan UGM, i-lib
collection	UGM
description	HBV replicates within the nucleus of hepatocyte using cellular transport machinery for the import of their genomes into the nucleus. Genome of HBV has to transported through the cytoplasm towards the nuclear pore complex (NPC) followed by subsequent passage through the pore. HBV capsid is involved in a number of important functions in the replication cycle of HBV. It can be detected in the nucleus, cytoplasm or both within infected hepatocytes. Nuclear localization of HBV capsid protein, which is karyophilic, depends on the cell cycle. The objective of the present study was to analyzes the intracellular localization of HBV capsid protein after transfected by entire HBV genome into hepatocyte cell lines (HuH-7) and to determine the predominantly localization of the capsid into cell compartment. In this work we analysed the intracellular localization of the HBV capsid in human hepatocyte cell lines liuH-7 by transfection using entire HBV genome and transient expression. The transfected cells were fixed and an indirect immune staining against the HBV capsid was performed to detect the capsid. To verify the location within the cell, an additional co-staining against the nuclear pore complexes was performed. The Intracellular localization of the HBV capsid and NPC were analyzed by a confocal laser scan microscope. The observed of localizations into the transfected cells were classified to be predominantly as nuclear localization, cytoplasmic localization or distributed within both of these compartments. Result of this study indicated that Staining of HBV capsid found predominantly within the nucleus (71%). Less frequently, the HBV capsid localized within the cytoplasm (26%). Only in a minority of cases, the capsids were localized within cytoplasm and nucleus (3%). This low frequency indicate that the capsids were not diffusing within the cells being in accordance to the in vivo situation in which the nuclear membrane was impermeable for the capsid. Key words: HBV capsid, hepatocyte, NPC, HuH-7, confocal laser scan microscope Replikasi HBV terjadi di dalam nukleus hepatosit dengan mempergunakan mekanisme transport seluler untuk mengirimkan genomnya ke dalam nukleus. Genom HBV hams ditransportasikan ke dalam nukleus melalui nuclear pore complex (NPC). Kapsid HBV terlibat dalam sejumlah fungsi panting dalam siklus replikasi HBV. Protein kapsid HBV dapat dideteksi didalam nukleus, sitoplasm ataupun di kedua kompartemen tersebut di dalam sel hepatosit yang terinfeksi HBV. Lokalisasi protein kapsid HBV yang bersifat karyophilik tergantung pada siklus sel. Adapun tujuan dari penelitian ini adalah untuk menganalisis lokalisasi intraseluler protein kapsid HBV pada sel line hepatosit HuH-7 pasca ditransfeksi dengan genom HBV utuh serta untuk menentukan lokalisasi kapsid HBV yang dominan dalam kompartemen sel. Pada penelitian telah dilakukan analisis lokalisasi intraseluler kapsid HBV dengan mentransfeksikan genome HBV utuh dan mengekspresikannya di dalam sel line HuH-7. Sel yang sudah ditransfeksi selanjumya difiksasi dan dilakukan penwarnaan secara tidak langsung terhadap kapsid HBV. Untuk menentukan lokalisasi dalam sel, pewarnaan lanjutan dilakukan terhadap NPC. Selanjutnya, dengan menggunakan mikroskop scan laser konfokal dianalisis lokalisasi intraseluler kapsid HBV dan NPC. Hasil pengamatan menunjukan bahwa distribusi HBV di dalam nukleus lebih dominan dibandingkan dengan distribusi di dalam sitoplasma dan distribusi pada kedua kompartemen tersebut. Secara kuantitatif menunjukkan bahwa distribusi protein kapsid HBV dalam nukleus adalah dominan dengan 71%, sedangkan dalam sitoplasma adalah 26%, sangat minoritas dijurnpai distribusi protein kapsid HBV pada kedua kompartemen sel, yaitu hanya 3%. Hal ini menunjukkan bahwa masuknya kapsid HBV kedalam nukleus tidak terdifusi secara pasif, namun secara aktif melalui mekanisme protein transport melalui NPC. Membran nukleus bersifat permeable terhadap protein HBV kapsid. Kata kunci: kapsid HBV, hepatosit, NPC, HuH-7, mikroskop scan laser konfocal
first_indexed	2024-03-05T23:07:35Z
format	Article
id	oai:generic.eprints.org:25638
institution	Universiti Gadjah Mada
last_indexed	2024-03-13T18:57:38Z
publishDate	2006
publisher	[Yogyakarta] : Universitas Gadjah Mada
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spelling	oai:generic.eprints.org:256382014-06-18T00:25:47Z https://repository.ugm.ac.id/25638/ Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV. Perpustakaan UGM, i-lib Jurnal i-lib UGM HBV replicates within the nucleus of hepatocyte using cellular transport machinery for the import of their genomes into the nucleus. Genome of HBV has to transported through the cytoplasm towards the nuclear pore complex (NPC) followed by subsequent passage through the pore. HBV capsid is involved in a number of important functions in the replication cycle of HBV. It can be detected in the nucleus, cytoplasm or both within infected hepatocytes. Nuclear localization of HBV capsid protein, which is karyophilic, depends on the cell cycle. The objective of the present study was to analyzes the intracellular localization of HBV capsid protein after transfected by entire HBV genome into hepatocyte cell lines (HuH-7) and to determine the predominantly localization of the capsid into cell compartment. In this work we analysed the intracellular localization of the HBV capsid in human hepatocyte cell lines liuH-7 by transfection using entire HBV genome and transient expression. The transfected cells were fixed and an indirect immune staining against the HBV capsid was performed to detect the capsid. To verify the location within the cell, an additional co-staining against the nuclear pore complexes was performed. The Intracellular localization of the HBV capsid and NPC were analyzed by a confocal laser scan microscope. The observed of localizations into the transfected cells were classified to be predominantly as nuclear localization, cytoplasmic localization or distributed within both of these compartments. Result of this study indicated that Staining of HBV capsid found predominantly within the nucleus (71%). Less frequently, the HBV capsid localized within the cytoplasm (26%). Only in a minority of cases, the capsids were localized within cytoplasm and nucleus (3%). This low frequency indicate that the capsids were not diffusing within the cells being in accordance to the in vivo situation in which the nuclear membrane was impermeable for the capsid. Key words: HBV capsid, hepatocyte, NPC, HuH-7, confocal laser scan microscope Replikasi HBV terjadi di dalam nukleus hepatosit dengan mempergunakan mekanisme transport seluler untuk mengirimkan genomnya ke dalam nukleus. Genom HBV hams ditransportasikan ke dalam nukleus melalui nuclear pore complex (NPC). Kapsid HBV terlibat dalam sejumlah fungsi panting dalam siklus replikasi HBV. Protein kapsid HBV dapat dideteksi didalam nukleus, sitoplasm ataupun di kedua kompartemen tersebut di dalam sel hepatosit yang terinfeksi HBV. Lokalisasi protein kapsid HBV yang bersifat karyophilik tergantung pada siklus sel. Adapun tujuan dari penelitian ini adalah untuk menganalisis lokalisasi intraseluler protein kapsid HBV pada sel line hepatosit HuH-7 pasca ditransfeksi dengan genom HBV utuh serta untuk menentukan lokalisasi kapsid HBV yang dominan dalam kompartemen sel. Pada penelitian telah dilakukan analisis lokalisasi intraseluler kapsid HBV dengan mentransfeksikan genome HBV utuh dan mengekspresikannya di dalam sel line HuH-7. Sel yang sudah ditransfeksi selanjumya difiksasi dan dilakukan penwarnaan secara tidak langsung terhadap kapsid HBV. Untuk menentukan lokalisasi dalam sel, pewarnaan lanjutan dilakukan terhadap NPC. Selanjutnya, dengan menggunakan mikroskop scan laser konfokal dianalisis lokalisasi intraseluler kapsid HBV dan NPC. Hasil pengamatan menunjukan bahwa distribusi HBV di dalam nukleus lebih dominan dibandingkan dengan distribusi di dalam sitoplasma dan distribusi pada kedua kompartemen tersebut. Secara kuantitatif menunjukkan bahwa distribusi protein kapsid HBV dalam nukleus adalah dominan dengan 71%, sedangkan dalam sitoplasma adalah 26%, sangat minoritas dijurnpai distribusi protein kapsid HBV pada kedua kompartemen sel, yaitu hanya 3%. Hal ini menunjukkan bahwa masuknya kapsid HBV kedalam nukleus tidak terdifusi secara pasif, namun secara aktif melalui mekanisme protein transport melalui NPC. Membran nukleus bersifat permeable terhadap protein HBV kapsid. Kata kunci: kapsid HBV, hepatosit, NPC, HuH-7, mikroskop scan laser konfocal [Yogyakarta] : Universitas Gadjah Mada 2006 Article NonPeerReviewed Perpustakaan UGM, i-lib (2006) Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8637
spellingShingle	Jurnal i-lib UGM Perpustakaan UGM, i-lib Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title	Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title_full	Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title_fullStr	Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title_full_unstemmed	Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title_short	Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.
title_sort	intracellular localization of hbv capsid in hepatocyte line after transfected by the entire hbv genome lokalisasi intraseluler kapsid hbv pada sel line hepatosit setelah ditransfeksi dengan genom utuh hbv
topic	Jurnal i-lib UGM
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Intracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV.

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