In Silico Approach of Glycinin and Conglycinin Chains of Soybean By-Product (Okara) Using Papain and Bromelain
This study explores utilization of a sustainable soybean by-product (okara) based on in silico approach. In silico approaches, as well as the BIOPEP database, PeptideRanker database, Peptide Calculator database (Pepcalc), ToxinPred database, and AllerTop database, were employed to evaluate the poten...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2022
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/278800/1/Ningrum_TP.pdf |
| _version_ | 1826050324211695616 |
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| author | Ningrum, Andriati Wardani, Dian Wahyu Vanidia, Nurul Sarifudin, Achmat Kumalasari, Rima Ekafitri, Riyanti Kristanti, Dita Setiaboma, Woro Munawaroh, Heli Siti Halimatul |
| author_facet | Ningrum, Andriati Wardani, Dian Wahyu Vanidia, Nurul Sarifudin, Achmat Kumalasari, Rima Ekafitri, Riyanti Kristanti, Dita Setiaboma, Woro Munawaroh, Heli Siti Halimatul |
| author_sort | Ningrum, Andriati |
| collection | UGM |
| description | This study explores utilization of a sustainable soybean by-product (okara) based on in silico approach. In silico approaches, as well as the BIOPEP database, PeptideRanker database, Peptide Calculator database (Pepcalc), ToxinPred database, and AllerTop database, were employed to evaluate the potential of glycinin and conglycinin derived peptides as a potential source of bioactive peptides. These major protein precursors have been found as protein in okara as a soybean by-product. Furthermore, primary structure, biological potential, and physicochemical, sensory, and allergenic characteristics of the theoretically released antioxidant peptides were predicted in this research.
Glycinin and α subunits of β-conglycinin were selected as potential precursors of bioactive peptides based on in silico analysis. The most notable among these are antioxidant peptides. First, the potential of protein precursors for releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are several antioxidant bioactive peptides in glycinin and β and α subunits of β-conglycinin sequences. Then, an in silico proteolysis using selected enzymes (papain, bromelain) to obtain antioxidant peptides was investigated and then analyzed using PeptideRanker and Pepcalc. Allergenic analysis using the AllerTop revealed that all in silico proteolysis-derived antioxidant peptides are probably
nonallergenic peptides. We also performed molecular docking against MPO (myeloperoxidases) for this peptide. Overall, the present study highlights that glycinin and β and α subunits of β-conglycinin could be promising precursors of bioactive peptides that have an antioxidant peptide for developing several applications. |
| first_indexed | 2024-03-14T00:02:10Z |
| format | Article |
| id | oai:generic.eprints.org:278800 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:02:10Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2788002023-11-02T00:26:07Z https://repository.ugm.ac.id/278800/ In Silico Approach of Glycinin and Conglycinin Chains of Soybean By-Product (Okara) Using Papain and Bromelain Ningrum, Andriati Wardani, Dian Wahyu Vanidia, Nurul Sarifudin, Achmat Kumalasari, Rima Ekafitri, Riyanti Kristanti, Dita Setiaboma, Woro Munawaroh, Heli Siti Halimatul Food Sciences Food Engineering This study explores utilization of a sustainable soybean by-product (okara) based on in silico approach. In silico approaches, as well as the BIOPEP database, PeptideRanker database, Peptide Calculator database (Pepcalc), ToxinPred database, and AllerTop database, were employed to evaluate the potential of glycinin and conglycinin derived peptides as a potential source of bioactive peptides. These major protein precursors have been found as protein in okara as a soybean by-product. Furthermore, primary structure, biological potential, and physicochemical, sensory, and allergenic characteristics of the theoretically released antioxidant peptides were predicted in this research. Glycinin and α subunits of β-conglycinin were selected as potential precursors of bioactive peptides based on in silico analysis. The most notable among these are antioxidant peptides. First, the potential of protein precursors for releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are several antioxidant bioactive peptides in glycinin and β and α subunits of β-conglycinin sequences. Then, an in silico proteolysis using selected enzymes (papain, bromelain) to obtain antioxidant peptides was investigated and then analyzed using PeptideRanker and Pepcalc. Allergenic analysis using the AllerTop revealed that all in silico proteolysis-derived antioxidant peptides are probably nonallergenic peptides. We also performed molecular docking against MPO (myeloperoxidases) for this peptide. Overall, the present study highlights that glycinin and β and α subunits of β-conglycinin could be promising precursors of bioactive peptides that have an antioxidant peptide for developing several applications. MDPI 2022-10-13 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/278800/1/Ningrum_TP.pdf Ningrum, Andriati and Wardani, Dian Wahyu and Vanidia, Nurul and Sarifudin, Achmat and Kumalasari, Rima and Ekafitri, Riyanti and Kristanti, Dita and Setiaboma, Woro and Munawaroh, Heli Siti Halimatul (2022) In Silico Approach of Glycinin and Conglycinin Chains of Soybean By-Product (Okara) Using Papain and Bromelain. Molecules, 2022 (27). pp. 1-11. ISSN 1420-3049 https://www.mdpi.com/journal/molecules https://doi.org/10.3390/molecules27206855 |
| spellingShingle | Food Sciences Food Engineering Ningrum, Andriati Wardani, Dian Wahyu Vanidia, Nurul Sarifudin, Achmat Kumalasari, Rima Ekafitri, Riyanti Kristanti, Dita Setiaboma, Woro Munawaroh, Heli Siti Halimatul In Silico Approach of Glycinin and Conglycinin Chains of Soybean By-Product (Okara) Using Papain and Bromelain |
| title | In Silico Approach of Glycinin and Conglycinin Chains
of Soybean By-Product (Okara) Using Papain and Bromelain |
| title_full | In Silico Approach of Glycinin and Conglycinin Chains
of Soybean By-Product (Okara) Using Papain and Bromelain |
| title_fullStr | In Silico Approach of Glycinin and Conglycinin Chains
of Soybean By-Product (Okara) Using Papain and Bromelain |
| title_full_unstemmed | In Silico Approach of Glycinin and Conglycinin Chains
of Soybean By-Product (Okara) Using Papain and Bromelain |
| title_short | In Silico Approach of Glycinin and Conglycinin Chains
of Soybean By-Product (Okara) Using Papain and Bromelain |
| title_sort | in silico approach of glycinin and conglycinin chains of soybean by product okara using papain and bromelain |
| topic | Food Sciences Food Engineering |
| url | https://repository.ugm.ac.id/278800/1/Ningrum_TP.pdf |
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