PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159
ABSTRACT A study was caffied out to characterize the cocoa lipase from cocoa beans (Theobroma cacao, L.) of clone PBC 159. The optimum temperature of cocoa lipase was 30-40 .C and the pH optimum was 7.0-8.0. The moleculer weight of the lipase enzyme was in between 45-66 kOa. The results indicate tha...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2008
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| _version_ | 1826033130013720576 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
A study was caffied out to characterize the cocoa lipase from cocoa beans (Theobroma cacao, L.) of clone
PBC 159. The optimum temperature of cocoa lipase was 30-40 .C and the pH optimum was 7.0-8.0. The moleculer
weight of the lipase enzyme was in between 45-66 kOa. The results indicate that Kmvalue for cocoa bean lipase was
2.63 mM, when trimyristin was used as a substrate. The incubation of cocoa bean lipase with triolein and tributyrin
(as substrate) yielded Kmof 11.24 and 35.71 mM, respectively. The Vmaxvalue obtained from the incubation of the
lipase with a wide range of substrates, including tributyrin, trimyristin and triolein, are expressed as pmole
acidlmin/mg protein for cocoa lipase. Vmax values decreased with the increase in the triacylglycerol chain-length, with
Vmax values of 27.78, 13.16 and 11.63 pmole acidlmin/mg protein when incubated with tributyrin, trimyristin and
triolein, respectively. Inhibition of lipase occurred in the presence of diisopropyl fIourophosphate, Nbromosuccinimide
and 5,5-dithiobis-(-2-nitrobenzoic acid).
Keywords: characterization, lipase, cocoa beans |
| first_indexed | 2024-03-13T19:05:18Z |
| format | Article |
| id | oai:generic.eprints.org:27943 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:05:18Z |
| publishDate | 2008 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:279432014-06-18T00:25:00Z https://repository.ugm.ac.id/27943/ PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT A study was caffied out to characterize the cocoa lipase from cocoa beans (Theobroma cacao, L.) of clone PBC 159. The optimum temperature of cocoa lipase was 30-40 .C and the pH optimum was 7.0-8.0. The moleculer weight of the lipase enzyme was in between 45-66 kOa. The results indicate that Kmvalue for cocoa bean lipase was 2.63 mM, when trimyristin was used as a substrate. The incubation of cocoa bean lipase with triolein and tributyrin (as substrate) yielded Kmof 11.24 and 35.71 mM, respectively. The Vmaxvalue obtained from the incubation of the lipase with a wide range of substrates, including tributyrin, trimyristin and triolein, are expressed as pmole acidlmin/mg protein for cocoa lipase. Vmax values decreased with the increase in the triacylglycerol chain-length, with Vmax values of 27.78, 13.16 and 11.63 pmole acidlmin/mg protein when incubated with tributyrin, trimyristin and triolein, respectively. Inhibition of lipase occurred in the presence of diisopropyl fIourophosphate, Nbromosuccinimide and 5,5-dithiobis-(-2-nitrobenzoic acid). Keywords: characterization, lipase, cocoa beans [Yogyakarta] : Universitas Gadjah Mada 2008 Article NonPeerReviewed Perpustakaan UGM, i-lib (2008) PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11006 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title | PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title_full | PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title_fullStr | PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title_full_unstemmed | PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title_short | PARTIAL CHARACTERIZATION OF LIPASE FROM COCOA BEANS (Theobroma cacao. L.) OF CLONE PBC 159 |
| title_sort | partial characterization of lipase from cocoa beans theobroma cacao l of clone pbc 159 |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib partialcharacterizationoflipasefromcocoabeanstheobromacacaolofclonepbc159 |