Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes
The primary virulence of soft rot pectobacteria, including Pectobacterium brasiliense, is mainly determined by the massive production of plant cell wall-degrading enzymes (PCWDEs), which promote plant tissue maceration in many crops. The antibiotic treatment to treat bacterial soft rot causes enviro...
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| Format: | Article |
| Language: | English |
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ASEAN Committee on Science and Technology
2022
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| Online Access: | https://repository.ugm.ac.id/283190/1/Manuka_Honey_Reduces_the_Virulence_of_Pectobacteri.pdf |
| _version_ | 1826050601273786368 |
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| author | Ava, Sheila Subandiyah, Siti Rohman, Muhammad Saifur Ogawa, Naoto Joko, Tri |
| author_facet | Ava, Sheila Subandiyah, Siti Rohman, Muhammad Saifur Ogawa, Naoto Joko, Tri |
| author_sort | Ava, Sheila |
| collection | UGM |
| description | The primary virulence of soft rot pectobacteria, including Pectobacterium brasiliense, is mainly determined by the massive production of plant cell wall-degrading enzymes (PCWDEs), which promote plant tissue maceration in many crops. The antibiotic treatment to treat bacterial soft rot causes environmental problems and potentially affects resistance. Antibiotic resistance is driving interest in antimicrobial treatments, and no organism has been reported to have acquired resistance to honey. However, the use of honey as a therapeutic agent for plant bacterial pathogens has rarely been studied. Therefore, this study was undertaken to determine the in vitro effect of Manuka honey against P. brasiliense at the phenotypic and genotypic levels. A sublethal concentration of honey was determined by a growth inhibition assay in broth medium containing different concentrations of Manuka honey. A macerating assay was performed on orchid leaves, and the activities of the PCWDEs were examined in plate assays. The expression of PCWDE-associated genes was investigated using semiquantitative reverse transcription-polymerase chain reaction analysis. The exposure of P. brasiliense to a sublethal concentration of Manuka honey significantly decreased the maceration ability of the orchid and the synthesis of PCWDEs, i.e., pectate lyase, polygalacturonase, and protease. Moreover, five PCWDEs-encoding genes, such as pelA, pelB, pelC, pehA, and prtW, had lower expression levels after the honey treatment compared with recA as the internal standard. The honey treatment decreased the virulence of P. brasiliense associated with the synthesis of PCWDEs. Therefore, Manuka honey reduced virulence by suppressing the expression of the PCWDE genes. © The Author(s) 2022. |
| first_indexed | 2024-03-14T00:06:58Z |
| format | Article |
| id | oai:generic.eprints.org:283190 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:06:58Z |
| publishDate | 2022 |
| publisher | ASEAN Committee on Science and Technology |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2831902023-11-21T00:29:56Z https://repository.ugm.ac.id/283190/ Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes Ava, Sheila Subandiyah, Siti Rohman, Muhammad Saifur Ogawa, Naoto Joko, Tri Crop and Pasture Protection (Pests Diseases and Weeds) The primary virulence of soft rot pectobacteria, including Pectobacterium brasiliense, is mainly determined by the massive production of plant cell wall-degrading enzymes (PCWDEs), which promote plant tissue maceration in many crops. The antibiotic treatment to treat bacterial soft rot causes environmental problems and potentially affects resistance. Antibiotic resistance is driving interest in antimicrobial treatments, and no organism has been reported to have acquired resistance to honey. However, the use of honey as a therapeutic agent for plant bacterial pathogens has rarely been studied. Therefore, this study was undertaken to determine the in vitro effect of Manuka honey against P. brasiliense at the phenotypic and genotypic levels. A sublethal concentration of honey was determined by a growth inhibition assay in broth medium containing different concentrations of Manuka honey. A macerating assay was performed on orchid leaves, and the activities of the PCWDEs were examined in plate assays. The expression of PCWDE-associated genes was investigated using semiquantitative reverse transcription-polymerase chain reaction analysis. The exposure of P. brasiliense to a sublethal concentration of Manuka honey significantly decreased the maceration ability of the orchid and the synthesis of PCWDEs, i.e., pectate lyase, polygalacturonase, and protease. Moreover, five PCWDEs-encoding genes, such as pelA, pelB, pelC, pehA, and prtW, had lower expression levels after the honey treatment compared with recA as the internal standard. The honey treatment decreased the virulence of P. brasiliense associated with the synthesis of PCWDEs. Therefore, Manuka honey reduced virulence by suppressing the expression of the PCWDE genes. © The Author(s) 2022. ASEAN Committee on Science and Technology 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283190/1/Manuka_Honey_Reduces_the_Virulence_of_Pectobacteri.pdf Ava, Sheila and Subandiyah, Siti and Rohman, Muhammad Saifur and Ogawa, Naoto and Joko, Tri (2022) Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes. ASEAN Journal on Science and Technology for Development, 39 (3). 119 – 124. ISSN 02175460 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145932873&doi=10.29037%2fajstd.880&partnerID=40&md5=5d149a51ae9d0ba155fddc6362e27093 10.29037/ajstd.880 |
| spellingShingle | Crop and Pasture Protection (Pests Diseases and Weeds) Ava, Sheila Subandiyah, Siti Rohman, Muhammad Saifur Ogawa, Naoto Joko, Tri Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title | Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title_full | Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title_fullStr | Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title_full_unstemmed | Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title_short | Manuka Honey Reduces the Virulence of Pectobacterium brasiliense by Suppressing Genes That Encode Plant Cell Wall-Degrading Enzymes |
| title_sort | manuka honey reduces the virulence of pectobacterium brasiliense by suppressing genes that encode plant cell wall degrading enzymes |
| topic | Crop and Pasture Protection (Pests Diseases and Weeds) |
| url | https://repository.ugm.ac.id/283190/1/Manuka_Honey_Reduces_the_Virulence_of_Pectobacteri.pdf |
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