Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection
Black rice as a functional food contains a high anthocyanin content, which functions as an antioxidant. However, bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has resulted in a reduction in yield. Black rice has been reported to have a higher resistance to BLB than white...
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| Format: | Article |
| Language: | English |
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by the Scientific Research and Innovation Support Fund, Ministry of Higher Education and Scientific Research, Jordan and published quarterly by the Deanship of Scientific Rese
2021
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| Online Access: | https://repository.ugm.ac.id/284200/1/Paper%20Number%2011.pdf |
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| author | Hidayah, Ema Nur Susanto, Febri Adi Joko, Tri Purwestri, Yekti Asih Nuringtyas, Tri Rini Atoum, Manar Fayiz Mousa Jan, Asad |
| author_facet | Hidayah, Ema Nur Susanto, Febri Adi Joko, Tri Purwestri, Yekti Asih Nuringtyas, Tri Rini Atoum, Manar Fayiz Mousa Jan, Asad |
| author_sort | Hidayah, Ema Nur |
| collection | UGM |
| description | Black rice as a functional food contains a high anthocyanin content, which functions as an antioxidant. However, bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has resulted in a reduction in yield. Black rice has been reported to have a higher resistance to BLB than white rice cultivars. This study aimed to determine the metabolite responses of two black rice cultivars, �Melik' and �Pari Ireng', compared to two white rice cultivars, �IR64' and �Java14', after infection with Xoo pathotype IV and mock negative control. Gas Chromatography-Mass Spectrometer (GC-MS) was used for the metabolite profiling analysis, followed by the quantification of total phenolic concentration (TPC) and total flavonoid concentration (TFC). Across all cultivars, the results revealed that following Xoo infection, there were significantly higher concentrations of primary metabolites including sugars, sugar alcohols, fatty acids, glycerol, and some organic acids. In �Pari Ireng' and �Melik' cultivars, sugars including fructose, and gluco-hexodialdose were significantly higher than the white cultivars following the infection. The infection significantly increased the TPC of all cultivars. �Java 14� contained the highest TPC while �Pari Ireng� was observed as cultivars with the highest TFC. These results suggested that pigmented rice cultivars possess a different chemical defense strategy from the white ones to respond to Xoo infection. © 2021 Jordan Journal of Biological Sciences. All Rights Reserved. |
| first_indexed | 2024-03-14T00:09:39Z |
| format | Article |
| id | oai:generic.eprints.org:284200 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:09:39Z |
| publishDate | 2021 |
| publisher | by the Scientific Research and Innovation Support Fund, Ministry of Higher Education and Scientific Research, Jordan and published quarterly by the Deanship of Scientific Rese |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2842002023-12-01T04:24:25Z https://repository.ugm.ac.id/284200/ Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection Hidayah, Ema Nur Susanto, Febri Adi Joko, Tri Purwestri, Yekti Asih Nuringtyas, Tri Rini Atoum, Manar Fayiz Mousa Jan, Asad Biological Sciences Black rice as a functional food contains a high anthocyanin content, which functions as an antioxidant. However, bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has resulted in a reduction in yield. Black rice has been reported to have a higher resistance to BLB than white rice cultivars. This study aimed to determine the metabolite responses of two black rice cultivars, �Melik' and �Pari Ireng', compared to two white rice cultivars, �IR64' and �Java14', after infection with Xoo pathotype IV and mock negative control. Gas Chromatography-Mass Spectrometer (GC-MS) was used for the metabolite profiling analysis, followed by the quantification of total phenolic concentration (TPC) and total flavonoid concentration (TFC). Across all cultivars, the results revealed that following Xoo infection, there were significantly higher concentrations of primary metabolites including sugars, sugar alcohols, fatty acids, glycerol, and some organic acids. In �Pari Ireng' and �Melik' cultivars, sugars including fructose, and gluco-hexodialdose were significantly higher than the white cultivars following the infection. The infection significantly increased the TPC of all cultivars. �Java 14� contained the highest TPC while �Pari Ireng� was observed as cultivars with the highest TFC. These results suggested that pigmented rice cultivars possess a different chemical defense strategy from the white ones to respond to Xoo infection. © 2021 Jordan Journal of Biological Sciences. All Rights Reserved. by the Scientific Research and Innovation Support Fund, Ministry of Higher Education and Scientific Research, Jordan and published quarterly by the Deanship of Scientific Rese 2021 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284200/1/Paper%20Number%2011.pdf Hidayah, Ema Nur and Susanto, Febri Adi and Joko, Tri and Purwestri, Yekti Asih and Nuringtyas, Tri Rini and Atoum, Manar Fayiz Mousa and Jan, Asad (2021) Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection. Jordan Journal of Biological Sciences, 14 (5). pp. 953-958. ISSN ISSN 1995-6673 https://jjbs.hu.edu.jo/files/vol14/n5/Paper%20Number%2011.pdf |
| spellingShingle | Biological Sciences Hidayah, Ema Nur Susanto, Febri Adi Joko, Tri Purwestri, Yekti Asih Nuringtyas, Tri Rini Atoum, Manar Fayiz Mousa Jan, Asad Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title | Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title_full | Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title_fullStr | Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title_full_unstemmed | Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title_short | Metabolite Profiling of Black Rice (Oryza sativa L.) Following Xanthomonas oryzae pv. oryzae Infection |
| title_sort | metabolite profiling of black rice oryza sativa l following xanthomonas oryzae pv oryzae infection |
| topic | Biological Sciences |
| url | https://repository.ugm.ac.id/284200/1/Paper%20Number%2011.pdf |
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