| Summary: | The experiment consisted of four stages. The experiment I was conducted to
determine the effect of CPO protection on hydrogenation of unsaturated fatty acid
contained in the diet by rumen microbes. The protection was done by mixing
expired date milk powder and CPO (2:1), added technical formaldehyde with
levels 0%, 1%, 2% and 3% formaldehyde to formed capsulated crude palm oil
(CCPO). In vitro test was done by using rumen fluid as a source of microbes,
elephant grass and rice bran (60:40) as substrates, and CCPO as feed additives as
much 15 mg. Results showed that the increasing of formaldehyde levels increased
concentration of oleic and linoleic acid (P<0.01). The experiment II was
conducted to determine the effect of CCPO addition in the diet on fatty acid
content, fermentation parameters and rumen microbial activity. In vitro test was
done by using rumen fluid as a source of microbes, elephant grass and rice bran
(60:40) as substrates, CPO additives as much 0.0%, 1.5%, 3.0%, 4.5% and 6.0%,
respectively protected with formaldehyde as much 0%, 1%, 2% and 3%. Results
showed that the increasing of formaldehyde levels increased fatty acid content
(P<0.05), CMC-ase activity, the number of protozoa (P<0.01) and microbial
protein (P<0.01). The increasing of CPO levels increased fatty acid content
(P<0.05) and microbial protein (P<0.01). The experiment III was conducted to
determine the effect of CCPO addition in the sheeps diet on fermentation
parameters, microbial activity, fatty acid content and in vivo digestibility.
Fifteenth local male lambs aged 9-12 months weighing 14-17 kg, were divided
into 3 groups ration treatment. The first group received only the basal ration (R0),
the 2nd group received the basal ration and 3% CPO (R1), while the 3rd group
received the basal ration and 3% CPO protected with 2% formaldehyde (R2). The
results showed that R2 treatment increased microbial protein (P<0.05) and
produce a better feed conversion (P<0.01). The experiment IV conducted to
determine the effect of CCPO addition in the ration on meat quality. The results
showed that R2 treatment increased meat protein and fat content (P<0.05), meat
tenderness (P<0.01) with a lower cooking loss (P<0.01) and unsaturated fatty acid
content was higher. From these results it could be concluded that the use of crude
palm oil protected with formaldehyde as an additive could improve the sheep
meat quality.
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