Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan
Lactic Acid Bacteria has been widely studied in dairy products. However, the presence of Lactic Acid Bacteria (LAB) in fruits has rarely been focused. Fruits contain organic acids and rich with nutrients such as minerals, carbohydrates, nitrogen compounds and a low pH environment that are suitable f...
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| Format: | Student Project |
| Language: | English |
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2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/32574/1/PPb_SITI%20MAS%20NUR%20AIN%20MAZLAN%20AS%20N%2017_5.pdf |
| _version_ | 1796903466362208256 |
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| author | Mazlan, Siti Mas Nur Ain |
| author_facet | Mazlan, Siti Mas Nur Ain |
| author_sort | Mazlan, Siti Mas Nur Ain |
| collection | UITM |
| description | Lactic Acid Bacteria has been widely studied in dairy products. However, the presence of Lactic Acid Bacteria (LAB) in fruits has rarely been focused. Fruits contain organic acids and rich with nutrients such as minerals, carbohydrates, nitrogen compounds and a low pH environment that are suitable for the growth of Lactobacillus sp. The objectives of this study were to isolate and identify Lactobacillus sp. from fresh fruits by using molecular techniques which is Polymerase Chain Reaction (PCR) method. The white colony from MRS agar was
isolated from banana and red dragon fruits. The phenotypic method was used to confirm the shapes and color of Lactic Acid Bacteria (LAB) which is Gram positive bacteria with rod or coccus shaped. The PCR results showed the identification of Lactobacillus sp. are at 216 bp by using the specific primer which is Lacto-16S-R and Lacto-16S-F. The results also indicate that Polymerase Chain Reaction (PCR) is more efficient to detect the strains of bacteria and give highly specific results. |
| first_indexed | 2024-03-06T02:20:36Z |
| format | Student Project |
| id | oai:ir.uitm.edu.my:32574 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T02:20:36Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:325742020-07-20T06:49:45Z https://ir.uitm.edu.my/id/eprint/32574/ Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan Mazlan, Siti Mas Nur Ain Extraction (Chemistry) Bacteria Lactic Acid Bacteria has been widely studied in dairy products. However, the presence of Lactic Acid Bacteria (LAB) in fruits has rarely been focused. Fruits contain organic acids and rich with nutrients such as minerals, carbohydrates, nitrogen compounds and a low pH environment that are suitable for the growth of Lactobacillus sp. The objectives of this study were to isolate and identify Lactobacillus sp. from fresh fruits by using molecular techniques which is Polymerase Chain Reaction (PCR) method. The white colony from MRS agar was isolated from banana and red dragon fruits. The phenotypic method was used to confirm the shapes and color of Lactic Acid Bacteria (LAB) which is Gram positive bacteria with rod or coccus shaped. The PCR results showed the identification of Lactobacillus sp. are at 216 bp by using the specific primer which is Lacto-16S-R and Lacto-16S-F. The results also indicate that Polymerase Chain Reaction (PCR) is more efficient to detect the strains of bacteria and give highly specific results. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/32574/1/PPb_SITI%20MAS%20NUR%20AIN%20MAZLAN%20AS%20N%2017_5.pdf Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan. (2017) [Student Project] (Unpublished) |
| spellingShingle | Extraction (Chemistry) Bacteria Mazlan, Siti Mas Nur Ain Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title | Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title_full | Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title_fullStr | Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title_full_unstemmed | Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title_short | Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan |
| title_sort | isolation and identification of lactobacillus sp from fresh fruits siti mas nur ain mazlan |
| topic | Extraction (Chemistry) Bacteria |
| url | https://ir.uitm.edu.my/id/eprint/32574/1/PPb_SITI%20MAS%20NUR%20AIN%20MAZLAN%20AS%20N%2017_5.pdf |
| work_keys_str_mv | AT mazlansitimasnurain isolationandidentificationoflactobacillusspfromfreshfruitssitimasnurainmazlan |