Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri

The herb plants are getting global attention and the Orthosiphon stamineus has been widely commercialized around the world into the tea product. However, the commercialization of O. stamineus is limited to its leaf only. This study aims to determine the phenolic and flavonoid content in flower, leaf...

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Main Author: Sabri, Fatin Syamimi
Format: Student Project
Language:English
Published: 2016
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/34668/1/34668.pdf
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author Sabri, Fatin Syamimi
author_facet Sabri, Fatin Syamimi
author_sort Sabri, Fatin Syamimi
collection UITM
description The herb plants are getting global attention and the Orthosiphon stamineus has been widely commercialized around the world into the tea product. However, the commercialization of O. stamineus is limited to its leaf only. This study aims to determine the phenolic and flavonoid content in flower, leaf, stem and root parts; and identify the part that has the highest content of phenolic and flavonoid. Phytochemical screening is done in which ferric chloride test for phenolic and alkaline reagent test for flavonoid. For the concentration, Folin-Ciocalteu method is for phenolic and aluminium chloride colorimetric assay is for flavonoid. As a result, the colour change of deep blue black appears indicating the presence of phenolic and the yellowish colour change appears indicating the presence of flavonoid. For concentration, the leaf shows the highest reading with 30.531±0.002 mg/ml for phenolic content and followed by flower, stem and root. For flavonoid content, the leaf has the highest content with 0.751±0.005 mg/ml and followed by flower, root and stem. The study proves that the leaf has the highest phenolic and flavonoid content with significant difference between the concentrations of the other parts.
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spelling oai:ir.uitm.edu.my:346682020-09-28T04:44:50Z https://ir.uitm.edu.my/id/eprint/34668/ Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri Sabri, Fatin Syamimi Laboratories. General works QR Microbiology The herb plants are getting global attention and the Orthosiphon stamineus has been widely commercialized around the world into the tea product. However, the commercialization of O. stamineus is limited to its leaf only. This study aims to determine the phenolic and flavonoid content in flower, leaf, stem and root parts; and identify the part that has the highest content of phenolic and flavonoid. Phytochemical screening is done in which ferric chloride test for phenolic and alkaline reagent test for flavonoid. For the concentration, Folin-Ciocalteu method is for phenolic and aluminium chloride colorimetric assay is for flavonoid. As a result, the colour change of deep blue black appears indicating the presence of phenolic and the yellowish colour change appears indicating the presence of flavonoid. For concentration, the leaf shows the highest reading with 30.531±0.002 mg/ml for phenolic content and followed by flower, stem and root. For flavonoid content, the leaf has the highest content with 0.751±0.005 mg/ml and followed by flower, root and stem. The study proves that the leaf has the highest phenolic and flavonoid content with significant difference between the concentrations of the other parts. 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34668/1/34668.pdf Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri. (2016) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
QR Microbiology
Sabri, Fatin Syamimi
Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title_full Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title_fullStr Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title_full_unstemmed Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title_short Identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus / Fatin Syamimi Sabri
title_sort identification of total phenolic and flavonoid content in different parts of orthosiphon stamineus fatin syamimi sabri
topic Laboratories. General works
QR Microbiology
url https://ir.uitm.edu.my/id/eprint/34668/1/34668.pdf
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