Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme
A selection of 36 commercial probiotic fermented dairy products from UK and Europe markets were evaluated for the numbers, types, and viability of Lactobacillus strains against the stated information on their packages. A comparative study was carried out on selectivity of MRS-Clindamycin, MRS-Sorbit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2021
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| Online Access: | https://repository.londonmet.ac.uk/6891/1/microorganisms-09-01600-v2.pdf |
| _version_ | 1825625603125018624 |
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| author | Farahmand, Nasim Ouoba, Irene Naghizadeh Raeisi, Shahram Sutherland, Jane P. Ghoddusi, Hamid |
| author_facet | Farahmand, Nasim Ouoba, Irene Naghizadeh Raeisi, Shahram Sutherland, Jane P. Ghoddusi, Hamid |
| author_sort | Farahmand, Nasim |
| collection | LMU |
| description | A selection of 36 commercial probiotic fermented dairy products from UK and Europe markets were evaluated for the numbers, types, and viability of Lactobacillus strains against the stated information on their packages. A comparative study was carried out on selectivity of MRS-Clindamycin, MRS-Sorbitol, and MRS-IM Maltose, to select the right medium for enumeration of probiotic Lactobacillus. Based on selectivity of medium for recovery of the targeted lactobacilli, and also simplicity of preparation, MRS-Clindamycin was chosen as the best medium for enumeration of probiotic Lactobacillus in fermented milks. The results of enumeration of lactobacilli showed that 22 out of a total 36 tested products contained more than 106 colony-forming units/g at the end of their shelf life, which comply with the recommended minimum therapeutic level for probiotics. Rep-PCR using primer GTG-5 was applied for initial discrimination of isolated strains, and isolates, which presented different band profile, were placed in different groups. The isolated Lactobacillus spp. were identified mainly as Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus paracasei by analysis of partial sequences of the 16S ribosomal RNA and rpoA genes. |
| first_indexed | 2024-07-09T04:02:42Z |
| format | Article |
| id | oai:repository.londonmet.ac.uk:6891 |
| institution | London Metropolitan University |
| language | English |
| last_indexed | 2024-07-09T04:02:42Z |
| publishDate | 2021 |
| publisher | MDPI |
| record_format | eprints |
| spelling | oai:repository.londonmet.ac.uk:68912021-08-06T09:51:56Z https://repository.londonmet.ac.uk/6891/ Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme Farahmand, Nasim Ouoba, Irene Naghizadeh Raeisi, Shahram Sutherland, Jane P. Ghoddusi, Hamid 570 Life sciences; biology A selection of 36 commercial probiotic fermented dairy products from UK and Europe markets were evaluated for the numbers, types, and viability of Lactobacillus strains against the stated information on their packages. A comparative study was carried out on selectivity of MRS-Clindamycin, MRS-Sorbitol, and MRS-IM Maltose, to select the right medium for enumeration of probiotic Lactobacillus. Based on selectivity of medium for recovery of the targeted lactobacilli, and also simplicity of preparation, MRS-Clindamycin was chosen as the best medium for enumeration of probiotic Lactobacillus in fermented milks. The results of enumeration of lactobacilli showed that 22 out of a total 36 tested products contained more than 106 colony-forming units/g at the end of their shelf life, which comply with the recommended minimum therapeutic level for probiotics. Rep-PCR using primer GTG-5 was applied for initial discrimination of isolated strains, and isolates, which presented different band profile, were placed in different groups. The isolated Lactobacillus spp. were identified mainly as Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus paracasei by analysis of partial sequences of the 16S ribosomal RNA and rpoA genes. MDPI 2021-07-27 Article PeerReviewed text en cc_by_4 https://repository.londonmet.ac.uk/6891/1/microorganisms-09-01600-v2.pdf Farahmand, Nasim, Ouoba, Irene, Naghizadeh Raeisi, Shahram, Sutherland, Jane P. and Ghoddusi, Hamid (2021) Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme. Microorganisms, 9 (8). pp. 1-16. ISSN 2076-2607 https://doi.org/10.3390/microorganisms9081600 10.3390/microorganisms9081600 10.3390/microorganisms9081600 |
| spellingShingle | 570 Life sciences; biology Farahmand, Nasim Ouoba, Irene Naghizadeh Raeisi, Shahram Sutherland, Jane P. Ghoddusi, Hamid Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title | Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title_full | Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title_fullStr | Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title_full_unstemmed | Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title_short | Probiotic Lactobacilli in fermented dairy products: selective detection, enumeration and identification scheme |
| title_sort | probiotic lactobacilli in fermented dairy products selective detection enumeration and identification scheme |
| topic | 570 Life sciences; biology |
| url | https://repository.londonmet.ac.uk/6891/1/microorganisms-09-01600-v2.pdf |
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