Active RNA polymerases: mobile or immobile molecular machines?
It is widely assumed that active RNA polymerases track along their templates to produce a transcript. We test this using chromosome conformation capture and human genes switched on rapidly and synchronously by tumour necrosis factor alpha (TNFalpha); one is 221 kbp SAMD4A, which a polymerase takes m...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1826256835347218432 |
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| author | Papantonis, A Larkin, J Wada, Y Ohta, Y Ihara, S Kodama, T Cook, P |
| author_facet | Papantonis, A Larkin, J Wada, Y Ohta, Y Ihara, S Kodama, T Cook, P |
| author_sort | Papantonis, A |
| collection | OXFORD |
| description | It is widely assumed that active RNA polymerases track along their templates to produce a transcript. We test this using chromosome conformation capture and human genes switched on rapidly and synchronously by tumour necrosis factor alpha (TNFalpha); one is 221 kbp SAMD4A, which a polymerase takes more than 1 h to transcribe. Ten minutes after stimulation, the SAMD4A promoter comes together with other TNFalpha-responsive promoters. Subsequently, these contacts are lost as new downstream ones appear; contacts are invariably between sequences being transcribed. Super-resolution microscopy confirms that nascent transcripts (detected by RNA fluorescence in situ hybridization) co-localize at relevant times. Results are consistent with an alternative view of transcription: polymerases fixed in factories reel in their respective templates, so different parts of the templates transiently lie together. |
| first_indexed | 2024-03-06T18:08:34Z |
| format | Journal article |
| id | oxford-uuid:023d4294-bc9a-484a-9d3f-733e014a9f7d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:08:34Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:023d4294-bc9a-484a-9d3f-733e014a9f7d2022-03-26T08:39:35ZActive RNA polymerases: mobile or immobile molecular machines?Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:023d4294-bc9a-484a-9d3f-733e014a9f7dEnglishSymplectic Elements at Oxford2010Papantonis, ALarkin, JWada, YOhta, YIhara, SKodama, TCook, PIt is widely assumed that active RNA polymerases track along their templates to produce a transcript. We test this using chromosome conformation capture and human genes switched on rapidly and synchronously by tumour necrosis factor alpha (TNFalpha); one is 221 kbp SAMD4A, which a polymerase takes more than 1 h to transcribe. Ten minutes after stimulation, the SAMD4A promoter comes together with other TNFalpha-responsive promoters. Subsequently, these contacts are lost as new downstream ones appear; contacts are invariably between sequences being transcribed. Super-resolution microscopy confirms that nascent transcripts (detected by RNA fluorescence in situ hybridization) co-localize at relevant times. Results are consistent with an alternative view of transcription: polymerases fixed in factories reel in their respective templates, so different parts of the templates transiently lie together. |
| spellingShingle | Papantonis, A Larkin, J Wada, Y Ohta, Y Ihara, S Kodama, T Cook, P Active RNA polymerases: mobile or immobile molecular machines? |
| title | Active RNA polymerases: mobile or immobile molecular machines? |
| title_full | Active RNA polymerases: mobile or immobile molecular machines? |
| title_fullStr | Active RNA polymerases: mobile or immobile molecular machines? |
| title_full_unstemmed | Active RNA polymerases: mobile or immobile molecular machines? |
| title_short | Active RNA polymerases: mobile or immobile molecular machines? |
| title_sort | active rna polymerases mobile or immobile molecular machines |
| work_keys_str_mv | AT papantonisa activernapolymerasesmobileorimmobilemolecularmachines AT larkinj activernapolymerasesmobileorimmobilemolecularmachines AT waday activernapolymerasesmobileorimmobilemolecularmachines AT ohtay activernapolymerasesmobileorimmobilemolecularmachines AT iharas activernapolymerasesmobileorimmobilemolecularmachines AT kodamat activernapolymerasesmobileorimmobilemolecularmachines AT cookp activernapolymerasesmobileorimmobilemolecularmachines |