Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy.

In vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों: Ovaa, H, van Swieten, P, Kessler, B, Leeuwenburgh, M, Fiebiger, E, van den Nieuwendijk, A, Galardy, P, van der Marel, G, Ploegh, H, Overkleeft, H
स्वरूप: Journal article
भाषा:English
प्रकाशित: 2003