Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy.
In vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797050870391635968 |
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| author | Ovaa, H van Swieten, P Kessler, B Leeuwenburgh, M Fiebiger, E van den Nieuwendijk, A Galardy, P van der Marel, G Ploegh, H Overkleeft, H |
| author_facet | Ovaa, H van Swieten, P Kessler, B Leeuwenburgh, M Fiebiger, E van den Nieuwendijk, A Galardy, P van der Marel, G Ploegh, H Overkleeft, H |
| author_sort | Ovaa, H |
| collection | OXFORD |
| description | In vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling of the catalytic activities of the proteasome in vivo. |
| first_indexed | 2024-03-06T18:11:37Z |
| format | Journal article |
| id | oxford-uuid:0338e030-dd59-417d-bb13-6d062b6edee1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:11:37Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:0338e030-dd59-417d-bb13-6d062b6edee12022-03-26T08:44:52ZChemistry in living cells: detection of active proteasomes by a two-step labeling strategy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0338e030-dd59-417d-bb13-6d062b6edee1EnglishSymplectic Elements at Oxford2003Ovaa, Hvan Swieten, PKessler, BLeeuwenburgh, MFiebiger, Evan den Nieuwendijk, AGalardy, Pvan der Marel, GPloegh, HOverkleeft, HIn vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling of the catalytic activities of the proteasome in vivo. |
| spellingShingle | Ovaa, H van Swieten, P Kessler, B Leeuwenburgh, M Fiebiger, E van den Nieuwendijk, A Galardy, P van der Marel, G Ploegh, H Overkleeft, H Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title | Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title_full | Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title_fullStr | Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title_full_unstemmed | Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title_short | Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. |
| title_sort | chemistry in living cells detection of active proteasomes by a two step labeling strategy |
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