Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction.
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultan...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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