A general method for preparing chromatin containing intact DNA.
A simple and general method is described for preparing chromatin from eukaryotic cells using isotonic conditions. First, cells are encapsulated in agarose microbeads and then lysed using Triton X-100 in the presence of a chelating agent and a physiological concentration of salt. Most cytoplasmic pro...
Main Authors: | , |
---|---|
Formato: | Journal article |
Idioma: | English |
Publicado em: |
1985
|