Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq.

Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and...

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Bibliographic Details
Main Authors: Haenni, S, Ji, Z, Hoque, M, Rust, N, Sharpe, H, Eberhard, R, Browne, C, Hengartner, M, Mellor, J, Tian, B, Furger, A
Format: Journal article
Language:English
Published: Oxford University Press 2012