Active genes are tri-methylated at K4 of histone H3.
Lysine methylation of histones in vivo occurs in three states: mono-, di- and tri-methyl. Histone H3 has been found to be di-methylated at lysine 4 (K4) in active euchromatic regions but not in silent heterochromatic sites. Here we show that the Saccharomyces cerevisiae Set1 protein can catalyse di-...
| Автори: | , , , , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
2002
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| _version_ | 1826258371160834048 |
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| author | Santos-Rosa, H Schneider, R Bannister, A Sherriff, J Bernstein, B Emre, N Schreiber, S Mellor, J Kouzarides, T |
| author_facet | Santos-Rosa, H Schneider, R Bannister, A Sherriff, J Bernstein, B Emre, N Schreiber, S Mellor, J Kouzarides, T |
| author_sort | Santos-Rosa, H |
| collection | OXFORD |
| description | Lysine methylation of histones in vivo occurs in three states: mono-, di- and tri-methyl. Histone H3 has been found to be di-methylated at lysine 4 (K4) in active euchromatic regions but not in silent heterochromatic sites. Here we show that the Saccharomyces cerevisiae Set1 protein can catalyse di- and tri-methylation of K4 and stimulate the activity of many genes. Using antibodies that discriminate between the di- and tri-methylated state of K4 we show that di-methylation occurs at both inactive and active euchromatic genes, whereas tri-methylation is present exclusively at active genes. It is therefore the presence of a tri-methylated K4 that defines an active state of gene expression. These findings establish the concept of methyl status as a determinant for gene activity and thus extend considerably the complexity of histone modifications. |
| first_indexed | 2024-03-06T18:32:56Z |
| format | Journal article |
| id | oxford-uuid:0a42a91f-5065-440c-9313-1fefd65e0938 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:32:56Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:0a42a91f-5065-440c-9313-1fefd65e09382022-03-26T09:22:57ZActive genes are tri-methylated at K4 of histone H3.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0a42a91f-5065-440c-9313-1fefd65e0938EnglishSymplectic Elements at Oxford2002Santos-Rosa, HSchneider, RBannister, ASherriff, JBernstein, BEmre, NSchreiber, SMellor, JKouzarides, TLysine methylation of histones in vivo occurs in three states: mono-, di- and tri-methyl. Histone H3 has been found to be di-methylated at lysine 4 (K4) in active euchromatic regions but not in silent heterochromatic sites. Here we show that the Saccharomyces cerevisiae Set1 protein can catalyse di- and tri-methylation of K4 and stimulate the activity of many genes. Using antibodies that discriminate between the di- and tri-methylated state of K4 we show that di-methylation occurs at both inactive and active euchromatic genes, whereas tri-methylation is present exclusively at active genes. It is therefore the presence of a tri-methylated K4 that defines an active state of gene expression. These findings establish the concept of methyl status as a determinant for gene activity and thus extend considerably the complexity of histone modifications. |
| spellingShingle | Santos-Rosa, H Schneider, R Bannister, A Sherriff, J Bernstein, B Emre, N Schreiber, S Mellor, J Kouzarides, T Active genes are tri-methylated at K4 of histone H3. |
| title | Active genes are tri-methylated at K4 of histone H3. |
| title_full | Active genes are tri-methylated at K4 of histone H3. |
| title_fullStr | Active genes are tri-methylated at K4 of histone H3. |
| title_full_unstemmed | Active genes are tri-methylated at K4 of histone H3. |
| title_short | Active genes are tri-methylated at K4 of histone H3. |
| title_sort | active genes are tri methylated at k4 of histone h3 |
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