GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells.
GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herei...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797052784828219392 |
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| author | Buza-Vidas, N Duarte, S Luc, S Bouriez-Jones, T Woll, P Jacobsen, SE |
| author_facet | Buza-Vidas, N Duarte, S Luc, S Bouriez-Jones, T Woll, P Jacobsen, SE |
| author_sort | Buza-Vidas, N |
| collection | OXFORD |
| description | GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal. |
| first_indexed | 2024-03-06T18:35:32Z |
| format | Journal article |
| id | oxford-uuid:0b16614d-21ea-4186-803f-a29faf0cd029 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:35:32Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:0b16614d-21ea-4186-803f-a29faf0cd0292022-03-26T09:27:33ZGATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0b16614d-21ea-4186-803f-a29faf0cd029EnglishSymplectic Elements at Oxford2011Buza-Vidas, NDuarte, SLuc, SBouriez-Jones, TWoll, PJacobsen, SEGATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal. |
| spellingShingle | Buza-Vidas, N Duarte, S Luc, S Bouriez-Jones, T Woll, P Jacobsen, SE GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title | GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title_full | GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title_fullStr | GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title_full_unstemmed | GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title_short | GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. |
| title_sort | gata3 is redundant for maintenance and self renewal of hematopoietic stem cells |
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