Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection.
Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity...
Main Authors: | , , , , |
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Format: | Journal article |
Language: | English |
Published: |
1990
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