Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection:  Evidence of Two-Step Photolysis.

The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of single-molecule detection (SMD) and for the image quality of scanning confocal microscopy. Concurrent results for the photostability were obtained by two different experimental techniques. First, the phot...

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Bibliographic Details
Main Authors: Eggeling, C, Widengren, J, Rigler, R, Seidel, C
Format: Journal article
Language:English
Published: 1998