Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection: Evidence of Two-Step Photolysis.
The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of single-molecule detection (SMD) and for the image quality of scanning confocal microscopy. Concurrent results for the photostability were obtained by two different experimental techniques. First, the phot...
Main Authors: | Eggeling, C, Widengren, J, Rigler, R, Seidel, C |
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Format: | Journal article |
Language: | English |
Published: |
1998
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