Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
Minisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA...
| Egile Nagusiak: | , , , , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
1987
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| _version_ | 1826258985562406912 |
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| author | Thein, S Jeffreys, A Gooi, H Cotter, F Flint, J O'Connor, N Weatherall, D Wainscoat, J |
| author_facet | Thein, S Jeffreys, A Gooi, H Cotter, F Flint, J O'Connor, N Weatherall, D Wainscoat, J |
| author_sort | Thein, S |
| collection | OXFORD |
| description | Minisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA in ten of the 35 cases. The changes included alterations in the relative intensities of hybridising DNA fragments, and, in three cases of cancers of gastrointestinal origin, the appearance of novel minisatellite fragments not seen in the corresponding constitutional DNA. The results of this preliminary study suggests that DNA fingerprint analysis provides a useful technique for identifying somatic changes in cancers. |
| first_indexed | 2024-03-06T18:42:43Z |
| format | Journal article |
| id | oxford-uuid:0d70f5e4-4152-4520-bbbb-4f8760f187aa |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:42:43Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:0d70f5e4-4152-4520-bbbb-4f8760f187aa2022-03-26T09:40:32ZDetection of somatic changes in human cancer DNA by DNA fingerprint analysis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0d70f5e4-4152-4520-bbbb-4f8760f187aaEnglishSymplectic Elements at Oxford1987Thein, SJeffreys, AGooi, HCotter, FFlint, JO'Connor, NWeatherall, DWainscoat, JMinisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA in ten of the 35 cases. The changes included alterations in the relative intensities of hybridising DNA fragments, and, in three cases of cancers of gastrointestinal origin, the appearance of novel minisatellite fragments not seen in the corresponding constitutional DNA. The results of this preliminary study suggests that DNA fingerprint analysis provides a useful technique for identifying somatic changes in cancers. |
| spellingShingle | Thein, S Jeffreys, A Gooi, H Cotter, F Flint, J O'Connor, N Weatherall, D Wainscoat, J Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title | Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title_full | Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title_fullStr | Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title_full_unstemmed | Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title_short | Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. |
| title_sort | detection of somatic changes in human cancer dna by dna fingerprint analysis |
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