Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18.
We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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