BirA enzyme: production and application in the study of membrane receptor-ligand interactions by site-specific biotinylation.
The enzyme BirA is a key reagent because of its ability to biotinylate proteins at a specific residue in a recognition sequence. We report a rapid, efficient, and economical method for the production, purification, and application of this enzyme. The method is easily scaled up and the protein produc...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Sprog: | English |
| Udgivet: |
1999
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