A simplified method of generating transgenic Xenopus

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of norma...

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Bibliographic Details
Main Authors: Sparrow, D, Latinkic, B, Mohun, T
Format: Journal article
Published: Oxford University Press 2000