A simplified method of generating transgenic Xenopus
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of norma...
Main Authors: | , , |
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Format: | Journal article |
Published: |
Oxford University Press
2000
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