A simplified method of generating transgenic Xenopus
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of norma...
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| Format: | Journal article |
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Oxford University Press
2000
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| _version_ | 1826259278210531328 |
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| author | Sparrow, D Latinkic, B Mohun, T |
| author_facet | Sparrow, D Latinkic, B Mohun, T |
| author_sort | Sparrow, D |
| collection | OXFORD |
| description | Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos. |
| first_indexed | 2024-03-06T18:47:20Z |
| format | Journal article |
| id | oxford-uuid:0ef4190f-0d8b-4c6d-bb7f-0da4fcc0ca90 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:47:20Z |
| publishDate | 2000 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:0ef4190f-0d8b-4c6d-bb7f-0da4fcc0ca902022-03-26T09:48:51ZA simplified method of generating transgenic XenopusJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0ef4190f-0d8b-4c6d-bb7f-0da4fcc0ca90Symplectic Elements at OxfordOxford University Press2000Sparrow, DLatinkic, BMohun, TCurrently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos. |
| spellingShingle | Sparrow, D Latinkic, B Mohun, T A simplified method of generating transgenic Xenopus |
| title | A simplified method of generating transgenic Xenopus |
| title_full | A simplified method of generating transgenic Xenopus |
| title_fullStr | A simplified method of generating transgenic Xenopus |
| title_full_unstemmed | A simplified method of generating transgenic Xenopus |
| title_short | A simplified method of generating transgenic Xenopus |
| title_sort | simplified method of generating transgenic xenopus |
| work_keys_str_mv | AT sparrowd asimplifiedmethodofgeneratingtransgenicxenopus AT latinkicb asimplifiedmethodofgeneratingtransgenicxenopus AT mohunt asimplifiedmethodofgeneratingtransgenicxenopus AT sparrowd simplifiedmethodofgeneratingtransgenicxenopus AT latinkicb simplifiedmethodofgeneratingtransgenicxenopus AT mohunt simplifiedmethodofgeneratingtransgenicxenopus |