Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation
Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Wiley
2016
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| _version_ | 1826307711119130624 |
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| author | Hölttä-Vuori, M Sezgin, E Eggeling, C Ikonen, E |
| author_facet | Hölttä-Vuori, M Sezgin, E Eggeling, C Ikonen, E |
| author_sort | Hölttä-Vuori, M |
| collection | OXFORD |
| description | Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling pattern of TF-Chol, similarly as with radiolabeled cholesterol. Lysosomal sterol accumulation characteristic to a lysosomal storage disease is most readily detected when the probe is introduced via the physiological route, i.e. as a sterol fatty acid ester in low-density lipoprotein particles. When administered to cells from solvent, lysosomal sterol sequestration becomes evident after an overnight equilibration between membranes. |
| first_indexed | 2024-03-07T07:07:12Z |
| format | Journal article |
| id | oxford-uuid:0f0a864e-e159-4795-b7e0-f24fa072fa17 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:07:12Z |
| publishDate | 2016 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:0f0a864e-e159-4795-b7e0-f24fa072fa172022-05-23T10:57:48ZUse of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0f0a864e-e159-4795-b7e0-f24fa072fa17EnglishSymplectic Elements at OxfordWiley2016Hölttä-Vuori, MSezgin, EEggeling, CIkonen, EDipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling pattern of TF-Chol, similarly as with radiolabeled cholesterol. Lysosomal sterol accumulation characteristic to a lysosomal storage disease is most readily detected when the probe is introduced via the physiological route, i.e. as a sterol fatty acid ester in low-density lipoprotein particles. When administered to cells from solvent, lysosomal sterol sequestration becomes evident after an overnight equilibration between membranes. |
| spellingShingle | Hölttä-Vuori, M Sezgin, E Eggeling, C Ikonen, E Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title | Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title_full | Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title_fullStr | Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title_full_unstemmed | Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title_short | Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation |
| title_sort | use of bodipy cholesterol tf chol for visualizing lysosomal cholesterol accumulation |
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