Looking for therapeutic antibodies in next-generation sequencing repositories
Recently it has become possible to query the great diversity of natural antibody repertoires using next-generation sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare clinical-stage therapeutic antibodies to the ~1b sequences from 60...
| Huvudupphovsmän: | , , , |
|---|---|
| Materialtyp: | Journal article |
| Publicerad: |
Taylor and Francis
2019
|
| _version_ | 1826259405580009472 |
|---|---|
| author | Krawczyk, K Raybould, M Kovaltsuk, A Deane, C |
| author_facet | Krawczyk, K Raybould, M Kovaltsuk, A Deane, C |
| author_sort | Krawczyk, K |
| collection | OXFORD |
| description | Recently it has become possible to query the great diversity of natural antibody repertoires using next-generation sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare clinical-stage therapeutic antibodies to the ~1b sequences from 60 independent sequencing studies in the Observed Antibody Space database, which includes antibody sequences from NGS analysis of immunoglobulin gene repertoires. Of 242 post-Phase 1 antibodies, we found 16 with sequence identity matches of 95% or better for both heavy and light chains. There are also 54 perfect matches to therapeutic CDR-H3 regions in the NGS outputs, suggesting a nontrivial amount of convergence between naturally observed sequences and those developed artificially. This has potential implications for both the legal protection of commercial antibodies and the discovery of antibody therapeutics. |
| first_indexed | 2024-03-06T18:49:21Z |
| format | Journal article |
| id | oxford-uuid:0fa7fc00-696e-414d-814b-a98a708fbb48 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:49:21Z |
| publishDate | 2019 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | oxford-uuid:0fa7fc00-696e-414d-814b-a98a708fbb482022-03-26T09:52:20ZLooking for therapeutic antibodies in next-generation sequencing repositoriesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0fa7fc00-696e-414d-814b-a98a708fbb48Symplectic Elements at OxfordTaylor and Francis2019Krawczyk, KRaybould, MKovaltsuk, ADeane, CRecently it has become possible to query the great diversity of natural antibody repertoires using next-generation sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare clinical-stage therapeutic antibodies to the ~1b sequences from 60 independent sequencing studies in the Observed Antibody Space database, which includes antibody sequences from NGS analysis of immunoglobulin gene repertoires. Of 242 post-Phase 1 antibodies, we found 16 with sequence identity matches of 95% or better for both heavy and light chains. There are also 54 perfect matches to therapeutic CDR-H3 regions in the NGS outputs, suggesting a nontrivial amount of convergence between naturally observed sequences and those developed artificially. This has potential implications for both the legal protection of commercial antibodies and the discovery of antibody therapeutics. |
| spellingShingle | Krawczyk, K Raybould, M Kovaltsuk, A Deane, C Looking for therapeutic antibodies in next-generation sequencing repositories |
| title | Looking for therapeutic antibodies in next-generation sequencing repositories |
| title_full | Looking for therapeutic antibodies in next-generation sequencing repositories |
| title_fullStr | Looking for therapeutic antibodies in next-generation sequencing repositories |
| title_full_unstemmed | Looking for therapeutic antibodies in next-generation sequencing repositories |
| title_short | Looking for therapeutic antibodies in next-generation sequencing repositories |
| title_sort | looking for therapeutic antibodies in next generation sequencing repositories |
| work_keys_str_mv | AT krawczykk lookingfortherapeuticantibodiesinnextgenerationsequencingrepositories AT raybouldm lookingfortherapeuticantibodiesinnextgenerationsequencingrepositories AT kovaltsuka lookingfortherapeuticantibodiesinnextgenerationsequencingrepositories AT deanec lookingfortherapeuticantibodiesinnextgenerationsequencingrepositories |