Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

BACKGROUND: When assessing the efficacy of gene transfer agents (GTAs) for cystic fibrosis (CF) gene therapy, we routinely evaluate gene transfer in the mouse nose and measure transfection efficiency by assessing transgene-specific mRNA using the real-time (TaqMan) quantitative reverse transcriptase...

Descripció completa

Dades bibliogràfiques
Autors principals:	Holder, E, Stevenson, B, Farley, R, Hilliard, T, Wodehouse, T, Somerton, L, Larsen, M, O'Donoghue, J, Coles, R, Scheule, R, Cheng, S, Gill, DR, Hyde, S, Griesenbach, U, Alton, E, Porteous, D, Boyd, A
Format:	Journal article
Idioma:	English
Publicat:	2010

Ítems similars

Assessment of CFTR function after gene transfer in vitro and in vivo.
per: Griesenbach, U, et al.
Publicat: (2008)

SAFETY AND EXPRESSION OF A SINGLE DOSE OF LIPID-MEDIATED CFTR GENE THERAPY TO THE UPPER AND LOWER AIRWAYS OF PATIENTS WITH CF
per: Davies, J, et al.
Publicat: (2011)

SAFETY AND EXPRESSION OF A SINGLE DOSE OF LIPID-MEDIATED CFTR GENE THERAPY TO THE UPPER AND LOWER AIRWAYS OF PATIENTS WITH CYSTIC FIBROSIS
per: Davies, G, et al.
Publicat: (2011)

Sendai virus-mediated CFTR gene transfer to the airway epithelium.
per: Ferrari, S, et al.
Publicat: (2007)

Self-reactive CFTR T cells in humans: implications for gene therapy.
per: Calcedo, R, et al.
Publicat: (2013)

REPEAT ADMINISTRATION OF GL67A/PGM169 IS FEASIBLE, SAFE, AND PRODUCES ENDOGENOUS LEVELS OF CFTR EXPRESSION AFTER 12 DOSES
per: Alton, E, et al.
Publicat: (2012)

Cumulative CFTR expression following repeated aerosol delivery of non-viral pGM169/GL67A formulation to mouse lung
per: Alton, E, et al.
Publicat: (2012)

Development of an immunocytochemical assay to detect human CFTR expression following gene therapy
per: Davidson, H, et al.
Publicat: (2008)

An immunocytochemical assay to detect human CFTR expression following gene transfer.
per: Davidson, H, et al.
Publicat: (2009)

LacZ siRNA and antisense DNA decrease beta-galactosidase mRNA but not protein expression in the airways of K18-lacZ transgenic mice
per: Griesenbach, U, et al.
Publicat: (2004)

Apical CFTR expression in human nasal epithelium correlates with lung disease in cystic fibrosis.
per: Marit Arianne van Meegen, et al.
Publicat: (2013-01-01)

Strategies for long-term expression of transgenes in the respiratory epithelium.
per: Gill, DR, et al.
Publicat: (2010)

CFTR, <it>P. aeruginosa</it> and apoptosis in respiratory epithelium
per: Burrows Lori, et al.
Publicat: (2000-10-01)

A PHASE 2B DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL OF NON-VIRAL MEDIATED GENE THERAPY FOR CF
per: Alton, E, et al.
Publicat: (2013)

A randomized, double-blind, placebo-controlled trial of repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis
per: Alton, E, et al.
Publicat: (2015)

TOXICOLOGY STUDIES IN SUPPORT OF THE UK CF GENE THERAPY CONSORTIUM'S MULTI-DOSE CLINICAL TRIAL
per: Griesenbach, U, et al.
Publicat: (2011)

Characterization of nasal potential difference in cftr knockout and F508del-CFTR mice.
per: Emilie Lyne Saussereau, et al.
Publicat: (2013-01-01)

Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice.
per: Davies, L, et al.
Publicat: (2008)

Down-regulation of the epithelial sodium channel ENaC through antisense oligonucleotides
per: Griesenbach, U, et al.
Publicat: (2004)

Assessment of the air-liquid interface culture model as a tool to validate efficacy of a rSIV.F/HN-CFTR vector
per: Sergijenko, A, et al.
Publicat: (2021)

Putting bicarbonate on the spot: pharmacological insights for CFTR correction in the airway epithelium
per: Miroslaw Zajac, et al.
Publicat: (2023-12-01)

Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium
per: Yukiko Sato, et al.
Publicat: (2021-12-01)

Pro-inflammatory cytokines stimulate CFTR-dependent anion secretion in pancreatic ductal epithelium
per: Dora Angyal, et al.
Publicat: (2024-01-01)

Low levels of CFTR gene transfer with F/HN pseudotyped lentivirus are sufficient to generate ion transport correction in airway cell cultures from cystic fibrosis patients
per: Sergijenko, A, et al.
Publicat: (2020)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung.
per: Alton, E, et al.
Publicat: (2014)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung
per: Alton, E, et al.
Publicat: (2014)

Optimizing nasal potential difference analysis for CFTR modulator development: assessment of ivacaftor in CF subjects with the G551D-CFTR mutation.
per: Steven M Rowe, et al.
Publicat: (2013-01-01)

Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo.
per: Griesenbach, U, et al.
Publicat: (2006)

Limitations of the murine nose in the development of nonviral airway gene transfer.
per: Griesenbach, U, et al.
Publicat: (2010)

DEVELOPMENT, PRODUCTION AND EVALUATION OF CLINICAL GRADE CFTR EXPRESSION PLASMID FOR CF LUNG GENE THERAPY
per: Gill, DR, et al.
Publicat: (2010)

Optimizing aerosol gene delivery and expression in the ovine lung.
per: McLachlan, G, et al.
Publicat: (2007)

Intestinal current measurement detects age-dependent differences in CFTR function in rectal epithelium
per: Simon Y. Graeber, et al.
Publicat: (2025-02-01)

Is CFTR-delF508 really absent from the apical membrane of the airway epithelium?
per: Lee A Borthwick, et al.
Publicat: (2011-01-01)

Novel vector improves efficiency of gene transfer to the airway epithelium
per: Pickles Raymond, et al.
Publicat: (2000-12-01)

The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways.
per: Griesenbach, U, et al.
Publicat: (2010)

CFTR expression analysis in human nasal epithelial cells by flow cytometry.
per: Marit A van Meegen, et al.
Publicat: (2011-01-01)

Correction of CFTR function in nasal epithelial cells from cystic fibrosis patients predicts improvement of respiratory function by CFTR modulators
per: Iwona M. Pranke, et al.
Publicat: (2017-08-01)

LONGITUDINAL ASSESSMENT OF BIOMARKERS FOR CLINICAL TRIALS OF NOVEL THERAPEUTIC AGENTS: THE RUN-IN STUDY
per: Alton, E, et al.
Publicat: (2010)

Non-viral CFTR gene delivery to the lungs of Cystic Fibrosis patients
per: Hyde, S
Publicat: (2013)

A randomised, double-blind, placebo-controlled phase IIB clinical trial of repeated application of gene therapy in patients with cystic fibrosis
per: Alton, E, et al.
Publicat: (2013)