Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex biological processes.
Fluorescence microscopy offers a minimally perturbative approach to probe biology in vivo. However, available techniques are limited both in sensitivity and temporal resolution for commonly used fluorescent proteins. Here we present a new imaging system with a diagnostic toolkit that caters for the...
Main Authors: | , , |
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Format: | Journal article |
Language: | English |
Published: |
2009
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