Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex biological processes.

Fluorescence microscopy offers a minimally perturbative approach to probe biology in vivo. However, available techniques are limited both in sensitivity and temporal resolution for commonly used fluorescent proteins. Here we present a new imaging system with a diagnostic toolkit that caters for the...

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Bibliographic Details
Main Authors: Plank, M, Wadhams, G, Leake, M
Format: Journal article
Language:English
Published: 2009