Single epitope multiple staining to detect ultralow frequency B cells.
Here we describe a method for detecting ultralow frequency target cells from within a high background of irrelevant cells by a novel method, single epitope multiple staining (SEMS). Samples of murine splenocytes were seeded with a low number of splenocytes from mice transgenic for a hen eggwhite lys...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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