Single epitope multiple staining to detect ultralow frequency B cells.
Here we describe a method for detecting ultralow frequency target cells from within a high background of irrelevant cells by a novel method, single epitope multiple staining (SEMS). Samples of murine splenocytes were seeded with a low number of splenocytes from mice transgenic for a hen eggwhite lys...
Main Authors: | , , |
---|---|
Formato: | Journal article |
Idioma: | English |
Publicado: |
2001
|