Single epitope multiple staining to detect ultralow frequency B cells.

Here we describe a method for detecting ultralow frequency target cells from within a high background of irrelevant cells by a novel method, single epitope multiple staining (SEMS). Samples of murine splenocytes were seeded with a low number of splenocytes from mice transgenic for a hen eggwhite lys...

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Detalles Bibliográficos
Main Authors: Townsend, SE, Goodnow, C, Cornall, R
Formato: Journal article
Idioma:English
Publicado: 2001