Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales
Medical countermeasures to treat biothreat agent infections require broad-spectrum therapeutics that do not induce agent resistance. A cell-based high-throughput screen (HTS) against ricin toxin combined with hit optimization allowed selection of a family of compounds that meet these requirements. T...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
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Elsevier
2016
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| _version_ | 1826260013900890112 |
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| author | Gupta, N Noël, R Goudet, A Hinsinger, K Michau, A Pons, V Abdelkafi, H Secher, T Shima, A Shtanko, O Sakurai, Y Cojean, S Pomel, S Liévin-Le Moal, V Leignel, V Herweg, J Fischer, A Johannes, L Harrison, K Beard, P Clayette, P Le Grand, R Rayner, J Rudel, T Vacus, J Loiseau, P Davey, R Oswald, E Cintrat, J Barbier, J Gillet, D |
| author_facet | Gupta, N Noël, R Goudet, A Hinsinger, K Michau, A Pons, V Abdelkafi, H Secher, T Shima, A Shtanko, O Sakurai, Y Cojean, S Pomel, S Liévin-Le Moal, V Leignel, V Herweg, J Fischer, A Johannes, L Harrison, K Beard, P Clayette, P Le Grand, R Rayner, J Rudel, T Vacus, J Loiseau, P Davey, R Oswald, E Cintrat, J Barbier, J Gillet, D |
| author_sort | Gupta, N |
| collection | OXFORD |
| description | Medical countermeasures to treat biothreat agent infections require broad-spectrum therapeutics that do not induce agent resistance. A cell-based high-throughput screen (HTS) against ricin toxin combined with hit optimization allowed selection of a family of compounds that meet these requirements. The hit compound Retro-2 and its derivatives have been demonstrated to be safe in vivo in mice even at high doses. Moreover, Retro-2 is an inhibitor of retrograde transport that affects syntaxin-5- dependent toxins and pathogens. As a consequence, it has a broad-spectrum activity that has been demonstrated both in vitro and in vivo against ricin, Shiga toxin-producing O104:H4 enterohemorrhagic E. coli and Leishmania sp. and in vitro against Ebola, Marburg and poxviruses and Chlamydiales. An effect is anticipated on other toxins or pathogens that use retrograde trafficking and syntaxin-5. Since Retro-2 targets cell components of the host and not directly the pathogen, no selection of resistant pathogens is expected. These lead compounds need now to be developed as drugs for human use. |
| first_indexed | 2024-03-06T18:58:55Z |
| format | Journal article |
| id | oxford-uuid:12d971f9-3452-450f-92a4-4b0d64664cbe |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:58:55Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:12d971f9-3452-450f-92a4-4b0d64664cbe2022-03-26T10:10:19ZInhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and ChlamydialesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:12d971f9-3452-450f-92a4-4b0d64664cbeSymplectic Elements at OxfordElsevier2016Gupta, NNoël, RGoudet, AHinsinger, KMichau, APons, VAbdelkafi, HSecher, TShima, AShtanko, OSakurai, YCojean, SPomel, SLiévin-Le Moal, VLeignel, VHerweg, JFischer, AJohannes, LHarrison, KBeard, PClayette, PLe Grand, RRayner, JRudel, TVacus, JLoiseau, PDavey, ROswald, ECintrat, JBarbier, JGillet, DMedical countermeasures to treat biothreat agent infections require broad-spectrum therapeutics that do not induce agent resistance. A cell-based high-throughput screen (HTS) against ricin toxin combined with hit optimization allowed selection of a family of compounds that meet these requirements. The hit compound Retro-2 and its derivatives have been demonstrated to be safe in vivo in mice even at high doses. Moreover, Retro-2 is an inhibitor of retrograde transport that affects syntaxin-5- dependent toxins and pathogens. As a consequence, it has a broad-spectrum activity that has been demonstrated both in vitro and in vivo against ricin, Shiga toxin-producing O104:H4 enterohemorrhagic E. coli and Leishmania sp. and in vitro against Ebola, Marburg and poxviruses and Chlamydiales. An effect is anticipated on other toxins or pathogens that use retrograde trafficking and syntaxin-5. Since Retro-2 targets cell components of the host and not directly the pathogen, no selection of resistant pathogens is expected. These lead compounds need now to be developed as drugs for human use. |
| spellingShingle | Gupta, N Noël, R Goudet, A Hinsinger, K Michau, A Pons, V Abdelkafi, H Secher, T Shima, A Shtanko, O Sakurai, Y Cojean, S Pomel, S Liévin-Le Moal, V Leignel, V Herweg, J Fischer, A Johannes, L Harrison, K Beard, P Clayette, P Le Grand, R Rayner, J Rudel, T Vacus, J Loiseau, P Davey, R Oswald, E Cintrat, J Barbier, J Gillet, D Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title | Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title_full | Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title_fullStr | Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title_full_unstemmed | Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title_short | Inhibitors of retrograde trafficking active against ricin and Shiga toxins also protect cells from several viruses, Leishmania and Chlamydiales |
| title_sort | inhibitors of retrograde trafficking active against ricin and shiga toxins also protect cells from several viruses leishmania and chlamydiales |
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