Mode of action and application of Scorpion primers to mutation detection.
Scorpion primers can be used to detect PCR products in homogeneous solution. Their structure promotes a unimolecular probing mechanism. We compare their performance with that of the same probe sequence forced to act in a bimolecular manner. The data suggest that Scorpions indeed probe by a unimolecu...
Main Authors: | , , , , |
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Format: | Journal article |
Language: | English |
Published: |
2000
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