Time-resolved fluorescence measurements using stroboscopic excitation

We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, an...

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Bibliographic Details
Main Authors: Matthews, DR, Summers, H, Njoh, K, Errington, R, Smith, P, Ameer-Beg, S, Vojnovic, B
Format: Journal article
Language:English
Published: 2005