Measuring nucleotide binding to intact, functional membrane proteins in real time
We have developed a method to measure binding of adenine nucleotides to intact, functional transmembrane receptors in a cellular or membrane environment. This method combines expression of proteins tagged with the fluorescent non-canonical amino acid ANAP, and FRET between ANAP and fluorescent (trin...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Journal of Visualized Experiments
2021
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