Measuring nucleotide binding to intact, functional membrane proteins in real time

Measuring nucleotide binding to intact, functional membrane proteins in real time

We have developed a method to measure binding of adenine nucleotides to intact, functional transmembrane receptors in a cellular or membrane environment. This method combines expression of proteins tagged with the fluorescent non-canonical amino acid ANAP, and FRET between ANAP and fluorescent (trin...

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Bibliographic Details
Main Authors:	Usher, S, Ashcroft, F, Puljung, M
Format:	Journal article
Language:	English
Published:	Journal of Visualized Experiments 2021

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