Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.
DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or bioti...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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