Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.
DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or bioti...
| Váldodahkkit: | , , |
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| Materiálatiipa: | Journal article |
| Giella: | English |
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2013
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| _version_ | 1826260438849945600 |
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| author | Li, W Gong, L Bayley, J |
| author_facet | Li, W Gong, L Bayley, J |
| author_sort | Li, W |
| collection | OXFORD |
| description | DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single-molecule level using protein nanopore analysis. Copyright © 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| first_indexed | 2024-03-06T19:05:43Z |
| format | Journal article |
| id | oxford-uuid:150b331e-e352-4c23-a27d-90a34e75e077 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:05:43Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:150b331e-e352-4c23-a27d-90a34e75e0772022-03-26T10:23:16ZSingle-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:150b331e-e352-4c23-a27d-90a34e75e077EnglishSymplectic Elements at Oxford2013Li, WGong, LBayley, JDNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single-molecule level using protein nanopore analysis. Copyright © 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| spellingShingle | Li, W Gong, L Bayley, J Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title | Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title_full | Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title_fullStr | Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title_full_unstemmed | Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title_short | Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. |
| title_sort | single molecule detection of 5 hydroxymethylcytosine in dna through chemical modification and nanopore analysis |
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