Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.

Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.

DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or bioti...

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Bibliographic Details
Main Authors:	Li, W, Gong, L, Bayley, J
Format:	Journal article
Language:	English
Published:	2013

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