MHC-peptide tetramers for the analysis of antigen-specific T cells.
The development of the fluorescently labeled tetrameric MHC-peptide complex has enabled the direct visualization, quantification and phenotypic characterization of antigen-specific T cells using flow cytometry and has transformed our understanding of cellular immune responses. The combination of thi...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1797055329671839744 |
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| author | Sims, S Willberg, C Klenerman, P |
| author_facet | Sims, S Willberg, C Klenerman, P |
| author_sort | Sims, S |
| collection | OXFORD |
| description | The development of the fluorescently labeled tetrameric MHC-peptide complex has enabled the direct visualization, quantification and phenotypic characterization of antigen-specific T cells using flow cytometry and has transformed our understanding of cellular immune responses. The combination of this technology with functional assays provides many new insights into these cells, allowing investigation into their lifecycle, manner of death and effector function. In this article, we hope to provide an overview of the techniques used in the construction of these tetramers, the problems and solutions associated with them, and the methods used in the study of antigen-specific T cells. Understanding how the antigen-specific cells develop and function in different circumstances and with different pathogens will be key to understanding natural host defense, as well as vaccine design and assessment. |
| first_indexed | 2024-03-06T19:09:09Z |
| format | Journal article |
| id | oxford-uuid:162d0daa-6e13-44bd-a412-75a3736c8c9e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:09:09Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:162d0daa-6e13-44bd-a412-75a3736c8c9e2022-03-26T10:29:49ZMHC-peptide tetramers for the analysis of antigen-specific T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:162d0daa-6e13-44bd-a412-75a3736c8c9eEnglishSymplectic Elements at Oxford2010Sims, SWillberg, CKlenerman, PThe development of the fluorescently labeled tetrameric MHC-peptide complex has enabled the direct visualization, quantification and phenotypic characterization of antigen-specific T cells using flow cytometry and has transformed our understanding of cellular immune responses. The combination of this technology with functional assays provides many new insights into these cells, allowing investigation into their lifecycle, manner of death and effector function. In this article, we hope to provide an overview of the techniques used in the construction of these tetramers, the problems and solutions associated with them, and the methods used in the study of antigen-specific T cells. Understanding how the antigen-specific cells develop and function in different circumstances and with different pathogens will be key to understanding natural host defense, as well as vaccine design and assessment. |
| spellingShingle | Sims, S Willberg, C Klenerman, P MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title | MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title_full | MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title_fullStr | MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title_full_unstemmed | MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title_short | MHC-peptide tetramers for the analysis of antigen-specific T cells. |
| title_sort | mhc peptide tetramers for the analysis of antigen specific t cells |
| work_keys_str_mv | AT simss mhcpeptidetetramersfortheanalysisofantigenspecifictcells AT willbergc mhcpeptidetetramersfortheanalysisofantigenspecifictcells AT klenermanp mhcpeptidetetramersfortheanalysisofantigenspecifictcells |