Active site mutations of recombinant deacetoxycephalosporin C synthase.

Site-directed mutagenesis of active site residues of deacetoxycephalosporin C synthase active site residues was carried out to investigate their role in catalysis. The following mutations were made and their effects on the conversion of 2-oxoglutarate and the oxidation of penicillin N or G were asse...

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Bibliographic Details
Main Authors: Lee, H, Schofield, C, Lloyd, MD
Format: Journal article
Language:English
Published: 2002