Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects.
Data from murine models of chronic viral infection suggest that CD4+ T-cell responses to viral pathogens are important in sustaining the number and/or function of CD8+ cytotoxic T-cell (CTL) effectors. In this study, we used cytokine flow cytometry (CFC), staining with HLA-A*0201-peptide tetramers,...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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| _version_ | 1826260722743508992 |
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| author | Komanduri, K Donahoe, S Moretto, W Schmidt, D Gillespie, G Ogg, G Roederer, M Nixon, D McCune, J |
| author_facet | Komanduri, K Donahoe, S Moretto, W Schmidt, D Gillespie, G Ogg, G Roederer, M Nixon, D McCune, J |
| author_sort | Komanduri, K |
| collection | OXFORD |
| description | Data from murine models of chronic viral infection suggest that CD4+ T-cell responses to viral pathogens are important in sustaining the number and/or function of CD8+ cytotoxic T-cell (CTL) effectors. In this study, we used cytokine flow cytometry (CFC), staining with HLA-A*0201-peptide tetramers, and peptide stimulation with epitopic peptides to study functional CD4+ and CD8+ T-cell responses to cytomegalovirus (CMV) in human subjects coinfected with CMV and the human immunodeficiency virus, type 1 (HIV-1). We show that strong CD4+ and CD8+ T-cell responses to CMV antigens are sustained over time in HIV-1-infected individuals. Those who maintain a strong CD4+ T-cell response to CMV are also likely to maintain higher frequencies of CD8+ T cells capable of binding to HLA-A*0201-CMV pp65 (A2-pp65) tetramers as well as responses to pp65 peptide stimulation with effector cytokine production. These data support the hypothesis that declines in frequencies of CD4+ T-cell responses to CMV are associated with an inability to sustain high levels of CMV-specific CD8+ T-cell responses in HIV-1-infected subjects. These declines may precede the onset of CMV-associated end organ disease. |
| first_indexed | 2024-03-06T19:10:12Z |
| format | Journal article |
| id | oxford-uuid:16831d6b-1ef5-43e0-8aad-60228965129a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:10:12Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:16831d6b-1ef5-43e0-8aad-60228965129a2022-03-26T10:31:44ZDirect measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:16831d6b-1ef5-43e0-8aad-60228965129aEnglishSymplectic Elements at Oxford2001Komanduri, KDonahoe, SMoretto, WSchmidt, DGillespie, GOgg, GRoederer, MNixon, DMcCune, JData from murine models of chronic viral infection suggest that CD4+ T-cell responses to viral pathogens are important in sustaining the number and/or function of CD8+ cytotoxic T-cell (CTL) effectors. In this study, we used cytokine flow cytometry (CFC), staining with HLA-A*0201-peptide tetramers, and peptide stimulation with epitopic peptides to study functional CD4+ and CD8+ T-cell responses to cytomegalovirus (CMV) in human subjects coinfected with CMV and the human immunodeficiency virus, type 1 (HIV-1). We show that strong CD4+ and CD8+ T-cell responses to CMV antigens are sustained over time in HIV-1-infected individuals. Those who maintain a strong CD4+ T-cell response to CMV are also likely to maintain higher frequencies of CD8+ T cells capable of binding to HLA-A*0201-CMV pp65 (A2-pp65) tetramers as well as responses to pp65 peptide stimulation with effector cytokine production. These data support the hypothesis that declines in frequencies of CD4+ T-cell responses to CMV are associated with an inability to sustain high levels of CMV-specific CD8+ T-cell responses in HIV-1-infected subjects. These declines may precede the onset of CMV-associated end organ disease. |
| spellingShingle | Komanduri, K Donahoe, S Moretto, W Schmidt, D Gillespie, G Ogg, G Roederer, M Nixon, D McCune, J Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title | Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title_full | Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title_fullStr | Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title_full_unstemmed | Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title_short | Direct measurement of CD4+ and CD8+ T-cell responses to CMV in HIV-1-infected subjects. |
| title_sort | direct measurement of cd4 and cd8 t cell responses to cmv in hiv 1 infected subjects |
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