Structural origins of FRET-observed nascent chain compaction on the ribosome

A fluorescence signal arising from a Förster resonance energy transfer process was used to monitor conformational changes of a domain within the E. coli protein HemK during its synthesis by the ribosome. An increase in fluorescence was observed to begin 10 s after translation was initiated, indicati...

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Bibliographic Details
Main Authors: Nissley, DA, O'Brien, EP
Format: Journal article
Language:English
Published: American Chemical Society 2018