SIMcheck: a toolbox for successful super-resolution structured illumination microscopy
Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to ident...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Nature Publishing Group
2015
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| _version_ | 1826261024388415488 |
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| author | Ball, G Demmerle, J Kaufmann, R Davis, I Dobbie, I Schermelleh, L |
| author_facet | Ball, G Demmerle, J Kaufmann, R Davis, I Dobbie, I Schermelleh, L |
| author_sort | Ball, G |
| collection | OXFORD |
| description | Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology. |
| first_indexed | 2024-03-06T19:15:02Z |
| format | Journal article |
| id | oxford-uuid:180c0af9-c3a6-41a6-8812-447ac434e967 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:15:02Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:180c0af9-c3a6-41a6-8812-447ac434e9672022-03-26T10:41:07ZSIMcheck: a toolbox for successful super-resolution structured illumination microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:180c0af9-c3a6-41a6-8812-447ac434e967EnglishSymplectic Elements at OxfordNature Publishing Group2015Ball, GDemmerle, JKaufmann, RDavis, IDobbie, ISchermelleh, LThree-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology. |
| spellingShingle | Ball, G Demmerle, J Kaufmann, R Davis, I Dobbie, I Schermelleh, L SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title | SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title_full | SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title_fullStr | SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title_full_unstemmed | SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title_short | SIMcheck: a toolbox for successful super-resolution structured illumination microscopy |
| title_sort | simcheck a toolbox for successful super resolution structured illumination microscopy |
| work_keys_str_mv | AT ballg simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy AT demmerlej simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy AT kaufmannr simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy AT davisi simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy AT dobbiei simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy AT schermellehl simcheckatoolboxforsuccessfulsuperresolutionstructuredilluminationmicroscopy |