Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change
Artificial protein cages are constructed from multiple protein subunits. The interaction between the subunits, notably the angle formed between them, controls the geometry of the resulting cage. Here, using the artificial protein cage, “TRAP-cage”, we show that a simple alteration in the position of...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2022
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| _version_ | 1797108149816131584 |
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| author | Sharma, M Biela, AP Kowalczyk, A Borzęcka-Solarz, K Piette, BMAG Gaweł, S Bishop, J Kukura, P Benesch, JLP Imamura, M Scheuring, S Heddle, JG |
| author_facet | Sharma, M Biela, AP Kowalczyk, A Borzęcka-Solarz, K Piette, BMAG Gaweł, S Bishop, J Kukura, P Benesch, JLP Imamura, M Scheuring, S Heddle, JG |
| author_sort | Sharma, M |
| collection | OXFORD |
| description | Artificial protein cages are constructed from multiple protein subunits. The interaction between the subunits, notably the angle formed between them, controls the geometry of the resulting cage. Here, using the artificial protein cage, “TRAP-cage”, we show that a simple alteration in the position of a single amino acid responsible for Au(I)-mediated subunit–subunit interactions in the constituent ring-shaped building blocks results in a more acute dihedral angle between them. In turn, this causes a dramatic shift in the structure from a 24-ring cage with an octahedral symmetry to a 20-ring cage with a C2 symmetry. This symmetry change is accompanied by a decrease in the number of Au(I)-mediated bonds between cysteines and a concomitant change in biophysical properties of the cage. |
| first_indexed | 2024-03-07T07:25:20Z |
| format | Journal article |
| id | oxford-uuid:18b392b9-ea59-44a1-a113-fe1e777158d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:25:20Z |
| publishDate | 2022 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:18b392b9-ea59-44a1-a113-fe1e777158d92022-11-15T10:28:44ZShape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid changeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:18b392b9-ea59-44a1-a113-fe1e777158d9EnglishSymplectic ElementsAmerican Chemical Society2022Sharma, MBiela, APKowalczyk, ABorzęcka-Solarz, KPiette, BMAGGaweł, SBishop, JKukura, PBenesch, JLPImamura, MScheuring, SHeddle, JGArtificial protein cages are constructed from multiple protein subunits. The interaction between the subunits, notably the angle formed between them, controls the geometry of the resulting cage. Here, using the artificial protein cage, “TRAP-cage”, we show that a simple alteration in the position of a single amino acid responsible for Au(I)-mediated subunit–subunit interactions in the constituent ring-shaped building blocks results in a more acute dihedral angle between them. In turn, this causes a dramatic shift in the structure from a 24-ring cage with an octahedral symmetry to a 20-ring cage with a C2 symmetry. This symmetry change is accompanied by a decrease in the number of Au(I)-mediated bonds between cysteines and a concomitant change in biophysical properties of the cage. |
| spellingShingle | Sharma, M Biela, AP Kowalczyk, A Borzęcka-Solarz, K Piette, BMAG Gaweł, S Bishop, J Kukura, P Benesch, JLP Imamura, M Scheuring, S Heddle, JG Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title | Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title_full | Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title_fullStr | Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title_full_unstemmed | Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title_short | Shape-morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
| title_sort | shape morphing of an artificial protein cage with unusual geometry induced by a single amino acid change |
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