Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

The implementation of high-throughput (HTP) cloning and expression screening in Escherichia coli by 14 laboratories in the Structural Proteomics In Europe (SPINE) consortium is described. Cloning efficiencies of greater than 80% have been achieved for the three non-ligation-based cloning techniques...

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Bibliographic Details
Main Authors:	Alzari, P, Berglund, H, Berrow, N, Blagova, E, Busso, D, Cambillau, C, Campanacci, V, Christodoulou, E, Eiler, S, Fogg, M, Folkers, G, Geerlof, A, Hart, D, Haouz, A, Herman, MD, Macieira, S, Nordlund, P, Perrakis, A, Quevillon-Cheruel, S, Tarandeau, F, van Tilbeurgh, H, Unger, T, Luna-Vargas, M, Velarde, M, Willmanns, M
Format:	Journal article
Language:	English
Published:	2006

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

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